Pub Date : 2025-11-26DOI: 10.1134/s1062359025602319
Sun Li, Jianbo Yang, Jian Jin
Fumonisin B1 (FB1), a water-soluble mycotoxin produced by Fusarium moniliforme, poses significant health risks to both humans and animals through widespread contamination of agricultural commodities. Paeoniflorin (PF), a bioactive monoterpene glycoside extracted from Paeonia lactiflora, exhibits potent anti-inflammatory and antioxidant properties. This study investigated the protective effects of PF against FB1-induced cytotoxicity in porcine alveolar macrophages (3D4/21) and explored the underlying molecular mechanisms. Cells were treated with FB1 (50 μg/mL) in combination with PF (100 μg/mL) for 24 h. Results demonstrated that PF significantly attenuated FB1-induced cytotoxicity by improving cell viability, restoring cytoskeletal and tight junction protein expression (α-Tubulin and ZO-1), and reducing apoptosis and oxidative stress. PF treatment successfully restored the balance of inflammatory cytokines (including IL-6, IL-8, IL-1β, IFN-α, and INF-γ) that had been dysregulated in 3D4/21 cells upon FB1 exposure. Transcriptomic analysis revealed that PF reversed FB1-induced gene expression alterations, particularly within clusters associated with cellular stress responses. Further investigation identified the p38 MAPK pathway as a critical mechanism through which PF exerts its protective effects, with significant downregulation of key pathway components including MAPK14, MAP2K3, ATF2, and MAPKAPK2 in cells co-treated with PF and FB1. These findings provide mechanistic insights into the protective effects of PF against environmental mycotoxins and suggest its potential application in mitigating FB1-induced agricultural and ecological toxicity.
{"title":"Paeoniflorin Attenuates Fumonisin B1-Induced Cytotoxicity by Inhibiting the p38 MAPK Pathway in 3D4/21 Cells","authors":"Sun Li, Jianbo Yang, Jian Jin","doi":"10.1134/s1062359025602319","DOIUrl":"https://doi.org/10.1134/s1062359025602319","url":null,"abstract":"Fumonisin B1 (FB1), a water-soluble mycotoxin produced by Fusarium moniliforme, poses significant health risks to both humans and animals through widespread contamination of agricultural commodities. Paeoniflorin (PF), a bioactive monoterpene glycoside extracted from Paeonia lactiflora, exhibits potent anti-inflammatory and antioxidant properties. This study investigated the protective effects of PF against FB1-induced cytotoxicity in porcine alveolar macrophages (3D4/21) and explored the underlying molecular mechanisms. Cells were treated with FB1 (50 μg/mL) in combination with PF (100 μg/mL) for 24 h. Results demonstrated that PF significantly attenuated FB1-induced cytotoxicity by improving cell viability, restoring cytoskeletal and tight junction protein expression (α-Tubulin and ZO-1), and reducing apoptosis and oxidative stress. PF treatment successfully restored the balance of inflammatory cytokines (including IL-6, IL-8, IL-1β, IFN-α, and INF-γ) that had been dysregulated in 3D4/21 cells upon FB1 exposure. Transcriptomic analysis revealed that PF reversed FB1-induced gene expression alterations, particularly within clusters associated with cellular stress responses. Further investigation identified the p38 MAPK pathway as a critical mechanism through which PF exerts its protective effects, with significant downregulation of key pathway components including MAPK14, MAP2K3, ATF2, and MAPKAPK2 in cells co-treated with PF and FB1. These findings provide mechanistic insights into the protective effects of PF against environmental mycotoxins and suggest its potential application in mitigating FB1-induced agricultural and ecological toxicity.","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":"52 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147914556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-09-04DOI: 10.1134/s1062359024610139
Menakshi Dhar, Seema Langer
Genetic analysis of any species depends upon the yield of DNA samples. In the present study, randomly amplified polymorphic DNA (RAPD-PCR) was optimized for the crab species inhabiting the freshwater ecosystems. In the initial step, the optimal concentration of PCR components and cycling conditions were standardized by altering the parameters of PCR reaction such as PCR buffer, magnesium chloride, dNTPs, primer, Taq polymerase, template DNA, denaturation time, annealing temperature and time, and volume of the reaction. Results indicated that the RAPD-PCR optimization system was 2 µL of 10X PCR buffer with 1.5 mM MgCl2, 2 µL of 2.5 mM dNTPs, 4 µL of primer (10 picomoles/µL), 0.25 µL of 5U Taq polymerase, 1 µL of template DNA (50 ng/µL) and nuclease-free water to make the total volume of 20 µL with denaturation time of 1 min at 94°C and an annealing temperature of 35°C. The optimized PCR and its programming conditions were then used to study the genetic diversity within the populations of freshwater crab, found in the different locations of the Jammu region. Assessment of genetic diversity among any species or the population of the species is essential for aquaculture practices.
任何物种的遗传分析都取决于DNA样本的产量。本研究利用随机扩增多态性DNA （RAPD-PCR）技术对生活在淡水生态系统中的蟹类进行了优化。在初始步骤中，通过改变PCR反应的参数，如PCR缓冲液、氯化镁、dNTPs、引物、Taq聚合酶、模板DNA、变性时间、退火温度和时间、反应体积等，标准化PCR组分的最佳浓度和循环条件。结果表明，RAPD-PCR优化体系为：10X PCR缓冲液2µL，含1.5 mM MgCl2, 2.5 mM dNTPs 2µL，引物4µL(10皮摩尔/µL)， 5U Taq聚合酶0.25µL，模板DNA 1µL (50 ng/µL)，无核酸酶水，使总体积为20µL，变性时间为1 min， 94℃，退火温度为35℃。利用优化后的PCR及其编程条件，对查谟地区不同地点淡水蟹种群的遗传多样性进行了研究。对任何物种或该物种种群的遗传多样性进行评估对水产养殖实践至关重要。
{"title":"Optimization of RAPD-PCR Assay for Assessing Genetic Variability in Brachyuran Crab Populations from Freshwater Ecosystems","authors":"Menakshi Dhar, Seema Langer","doi":"10.1134/s1062359024610139","DOIUrl":"https://doi.org/10.1134/s1062359024610139","url":null,"abstract":"Genetic analysis of any species depends upon the yield of DNA samples. In the present study, randomly amplified polymorphic DNA (RAPD-PCR) was optimized for the crab species inhabiting the freshwater ecosystems. In the initial step, the optimal concentration of PCR components and cycling conditions were standardized by altering the parameters of PCR reaction such as PCR buffer, magnesium chloride, dNTPs, primer, Taq polymerase, template DNA, denaturation time, annealing temperature and time, and volume of the reaction. Results indicated that the RAPD-PCR optimization system was 2 µL of 10X PCR buffer with 1.5 mM MgCl2, 2 µL of 2.5 mM dNTPs, 4 µL of primer (10 picomoles/µL), 0.25 µL of 5U Taq polymerase, 1 µL of template DNA (50 ng/µL) and nuclease-free water to make the total volume of 20 µL with denaturation time of 1 min at 94°C and an annealing temperature of 35°C. The optimized PCR and its programming conditions were then used to study the genetic diversity within the populations of freshwater crab, found in the different locations of the Jammu region. Assessment of genetic diversity among any species or the population of the species is essential for aquaculture practices.","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":"52 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147904649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-08-01DOI: 10.1134/s1062359024613533
S. Rajput, S. Singh, Kanad Das, Yash Pal Sharma
During an extensive and regular survey conducted in the less-explored coniferous forests of Jammu, India, some interesting specimens of boletoid mushrooms were collected. Thorough morphological characterisation followed by two-gene molecular phylogenetic analyses of two specimens, revealed two interesting species: Rubroboletus himalayensis and Phylloporus nigrisquamus, representing first report from India. Their detailed descriptions, illustrations, phylogenetic inferences, and comprehensive comparisons with corresponding similar taxa are also discussed.
{"title":"Two New Records of Boletoid Mushrooms (Boletaceae) from India","authors":"S. Rajput, S. Singh, Kanad Das, Yash Pal Sharma","doi":"10.1134/s1062359024613533","DOIUrl":"https://doi.org/10.1134/s1062359024613533","url":null,"abstract":"During an extensive and regular survey conducted in the less-explored coniferous forests of Jammu, India, some interesting specimens of boletoid mushrooms were collected. Thorough morphological characterisation followed by two-gene molecular phylogenetic analyses of two specimens, revealed two interesting species: Rubroboletus himalayensis and Phylloporus nigrisquamus, representing first report from India. Their detailed descriptions, illustrations, phylogenetic inferences, and comprehensive comparisons with corresponding similar taxa are also discussed.","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":"52 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147885334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-08-01DOI: 10.1134/s1062359024610942
Yichen Liu, Sunan Xu, Xuemei Li, Lihong Zhang
Pb and Pyrene, prevalent in soil, significantly affect plants. Ascorbate-glutathione (AsA-GSH) cycle is crucial for plant antioxidant defense mechanisms. Understanding the clonal integration of clonal plants in heterogeneous contaminated soil is important. This work investigates the potential effects of clonal integration on the key players in specifically the metabolism, AsA-GSH cycle, in Zoysia japonica ramets under three heterogeneous stresses (Pb and/or Pyrene) and two stolon connection statuses (connected and disconnected ramets). The results showed that connected stressed ramets exhibit higher biomass accumulation and lower H2O2 content compared to disconnected stressed ramets. This can be attributed to the enhancement of Ascorbate Peroxidase (APX) enzyme activity, the preservation of Glutathione Reductase (GR), Dehydroascorbate Reductase (DHAR), and Monodehydroascorbate Reductase (MDHAR) activity. Furthermore, there was a reduction in the decline of Ascorbic Acid (AsA) and Glutathione (GSH), and a decrease in the rise of Dehydroascorbic Acid (DHA) and Glutathione Disulfide (GSSG). Among three heterogeneous stresses, clonal integration performs better under Pb or Pyrene stress than under Pb + Pyrene stress. Our study highlights the vital role of clonal integration in maintaining redox balance and supporting the success of clonal plants in toxic environments, even under severe combined stress.
{"title":"Clonal Integration between Zoysia Ramets Improves Overall Resistance under Heterogeneous Pb and/or Pyrene Stresses by Modulating AsA-GSH Cycle","authors":"Yichen Liu, Sunan Xu, Xuemei Li, Lihong Zhang","doi":"10.1134/s1062359024610942","DOIUrl":"https://doi.org/10.1134/s1062359024610942","url":null,"abstract":"Pb and Pyrene, prevalent in soil, significantly affect plants. Ascorbate-glutathione (AsA-GSH) cycle is crucial for plant antioxidant defense mechanisms. Understanding the clonal integration of clonal plants in heterogeneous contaminated soil is important. This work investigates the potential effects of clonal integration on the key players in specifically the metabolism, AsA-GSH cycle, in Zoysia japonica ramets under three heterogeneous stresses (Pb and/or Pyrene) and two stolon connection statuses (connected and disconnected ramets). The results showed that connected stressed ramets exhibit higher biomass accumulation and lower H2O2 content compared to disconnected stressed ramets. This can be attributed to the enhancement of Ascorbate Peroxidase (APX) enzyme activity, the preservation of Glutathione Reductase (GR), Dehydroascorbate Reductase (DHAR), and Monodehydroascorbate Reductase (MDHAR) activity. Furthermore, there was a reduction in the decline of Ascorbic Acid (AsA) and Glutathione (GSH), and a decrease in the rise of Dehydroascorbic Acid (DHA) and Glutathione Disulfide (GSSG). Among three heterogeneous stresses, clonal integration performs better under Pb or Pyrene stress than under Pb + Pyrene stress. Our study highlights the vital role of clonal integration in maintaining redox balance and supporting the success of clonal plants in toxic environments, even under severe combined stress.","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":"52 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147331390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-07-26DOI: 10.1134/s1062359024612679
Mostafa Alamholo, Aladdin Sheikhmous Murad
Transcription factors (TFs) are a group of proteins that regulate drought stress-responsive genes by binding to DNA at specific elements in promoter regions. No combinatorial analysis has been performed to identify the TFs and gene ontology (GO) involved in drought stress tolerance so far. Soybean microarray datasets were analyzed through R language packages. To identify TFs, sequences of down and up-regulated genes involved in drought stress tolerance using multiple promoter analysis was used. Common GO down and up-regulated genes were identified by Venny v2.1. Up-regulated key TFs were associated with AT-Hook, homeodomain, MYb/SANT, TBP, and WRKY and down-regulated key TFs related to Dof, bHLH, bZIP, and ZF-HD. Biological processes (BP) of up-regulated genes mainly belonged to fatty acid and photosynthesis processes and down-regulated genes mostly related to response to abiotic stresses. Molecular function (MF) of up-regulated genes mainly related to transferase enzymes and down-regulated genes mostly connected to regulators and inhibitory enzymes. Based on the results of this study, it is possible to understand the molecular mechanisms related to drought stress in soybean, and key TFs can be used to control the expression of genes related to drought stress in plant breeding and genetic engineering.
{"title":"Gene Ontology and Identification of Transcription Factors Related to Key Genes Involved in Drought Stress Tolerance of Soybean (Glycine max L.)","authors":"Mostafa Alamholo, Aladdin Sheikhmous Murad","doi":"10.1134/s1062359024612679","DOIUrl":"https://doi.org/10.1134/s1062359024612679","url":null,"abstract":"Transcription factors (TFs) are a group of proteins that regulate drought stress-responsive genes by binding to DNA at specific elements in promoter regions. No combinatorial analysis has been performed to identify the TFs and gene ontology (GO) involved in drought stress tolerance so far. Soybean microarray datasets were analyzed through R language packages. To identify TFs, sequences of down and up-regulated genes involved in drought stress tolerance using multiple promoter analysis was used. Common GO down and up-regulated genes were identified by Venny v2.1. Up-regulated key TFs were associated with AT-Hook, homeodomain, MYb/SANT, TBP, and WRKY and down-regulated key TFs related to Dof, bHLH, bZIP, and ZF-HD. Biological processes (BP) of up-regulated genes mainly belonged to fatty acid and photosynthesis processes and down-regulated genes mostly related to response to abiotic stresses. Molecular function (MF) of up-regulated genes mainly related to transferase enzymes and down-regulated genes mostly connected to regulators and inhibitory enzymes. Based on the results of this study, it is possible to understand the molecular mechanisms related to drought stress in soybean, and key TFs can be used to control the expression of genes related to drought stress in plant breeding and genetic engineering.","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":"52 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147885713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In the serum exosomes of hepatocellular carcinoma (HCC) patients, miR-4661-5p is reported at a high level. This study aimed to investigate the effect of exosomal miR-4661-5p on the malignant progression of HCC and its mechanism of action. Exosomes were separated from SNU387 cells and identified and miR-4661-5p expression were tested using qRT-PCR. CCK-8, clone formation, and Transwell assays tested the biological characteristics of HCC cells, and flow cytometry determined apoptosis rate. Dual-luciferase reporter gene tests verified the targeting connection between miR-4661-5p and Estrogen receptor alpha (ESR1). Moreover, a subcutaneous graft tumor model was constructed in nude mice. MiR-4661-5p exhibited an elevated level in HCC cells and their exosomes; ESR1 exhibited a notably diminished level in HCC cells; miR-4661-5p targeted and negatively regulated ESR1. After co-culturing with exosomes, Hep3B and Huh7 cells showed heightened levels of miR-4661-5p. Exosomal miR-4661-5p promoted the migration and invasion of HCC cells and depressed apoptosis. Overexpression ESR1 partially declined the promoting impact of over-expressed exosomal miR-4661-5p on the proliferation and invasion ability of Hep3B and Huh7 cells and the suppression of apoptosis. In addition, exosomal or over-expressed miR-4661-5p accelerated tumor expansion in nude mice by regulating the level of ESR1. In conclusion, exosomal miR-4661-5p targets and downregulates ESR1, leading to the promotion of HCC malignant progression, identified exosomal miR-4661-5p and ESR1 as potential therapeutic targets for HCC.
{"title":"Tumor-Derived Exosome miR-4661-5p Exerts Tumor-Promoting Effects in Hepatocellular Carcinoma by Targeting ESR1","authors":"Sha Li, Qian Wang, Lixiu Xu, Mengmeng Liu, Yanni Ma, Wensheng Liu","doi":"10.1134/s1062359024612230","DOIUrl":"https://doi.org/10.1134/s1062359024612230","url":null,"abstract":"In the serum exosomes of hepatocellular carcinoma (HCC) patients, miR-4661-5p is reported at a high level. This study aimed to investigate the effect of exosomal miR-4661-5p on the malignant progression of HCC and its mechanism of action. Exosomes were separated from SNU387 cells and identified and miR-4661-5p expression were tested using qRT-PCR. CCK-8, clone formation, and Transwell assays tested the biological characteristics of HCC cells, and flow cytometry determined apoptosis rate. Dual-luciferase reporter gene tests verified the targeting connection between miR-4661-5p and Estrogen receptor alpha (ESR1). Moreover, a subcutaneous graft tumor model was constructed in nude mice. MiR-4661-5p exhibited an elevated level in HCC cells and their exosomes; ESR1 exhibited a notably diminished level in HCC cells; miR-4661-5p targeted and negatively regulated ESR1. After co-culturing with exosomes, Hep3B and Huh7 cells showed heightened levels of miR-4661-5p. Exosomal miR-4661-5p promoted the migration and invasion of HCC cells and depressed apoptosis. Overexpression ESR1 partially declined the promoting impact of over-expressed exosomal miR-4661-5p on the proliferation and invasion ability of Hep3B and Huh7 cells and the suppression of apoptosis. In addition, exosomal or over-expressed miR-4661-5p accelerated tumor expansion in nude mice by regulating the level of ESR1. In conclusion, exosomal miR-4661-5p targets and downregulates ESR1, leading to the promotion of HCC malignant progression, identified exosomal miR-4661-5p and ESR1 as potential therapeutic targets for HCC.","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":"52 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147922076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-06-01DOI: 10.1134/s1062359024612436
Junrong Zhang, Chong Zhao
Armillaria mellea is a valuable medicinal and edible fungus widely used in China for its health-promoting properties. It exhibits a broad spectrum of pharmacological activities. Extracts from Armillaria mellea have demonstrated cytotoxic effects against a variety of cancer cell lines. These extracts induce apoptosis and inhibit cell proliferation through multiple mechanisms, including the modulation of critical signaling pathways involved in cell survival and programmed cell death. An additional notable advantage of Armillaria mellea is its non-toxicity, particularly in comparison to conventional chemotherapeutic agents, which frequently lead to adverse side effects. The present study aimed to investigate the anti-lung cancer effects of Armillaria mellea mycelium aqueous (AMMA) extract in the A549 cell line. MTT assay, Hoechst 33342, Fluo-4-AM staining, DCFH-DA staining and ELISA were utilized to evaluate changes in cell viability, nuclear apoptosis, Ca2+ concentration, intracellular reactive oxygen species (ROS) concentration, lactate dehydrogenase (LDH), malondialdehyde (MDA), catalase (CAT), superoxide dismutase (SOD), and the protein expression levels of Caspase-3/-8/-9. The results demonstrated that AMMA effectively reduced the survival rate of A549 cells, promoted nuclear apoptosis, calcium ion, intracellular ROS level, LDH and MDA content, inhibited high levels of CAT and SOD, and activated the Caspase-3/-8/-9 apoptosis pathway, ultimately mediating the death of cancer cells. This study confirmed that AMMA has an effective anti-lung cancer activity via the Caspase-3/-8/-9 pathway. These data also clarify the potential of AMMA as a natural product based therapeutic agent for the treatment of non-small cell lung cancer.
{"title":"Armillaria mellea Mycelium Aqueous Extract-Induced Apoptosis in A549 Cells via a Caspase-Mediated Pathway","authors":"Junrong Zhang, Chong Zhao","doi":"10.1134/s1062359024612436","DOIUrl":"https://doi.org/10.1134/s1062359024612436","url":null,"abstract":"Armillaria mellea is a valuable medicinal and edible fungus widely used in China for its health-promoting properties. It exhibits a broad spectrum of pharmacological activities. Extracts from Armillaria mellea have demonstrated cytotoxic effects against a variety of cancer cell lines. These extracts induce apoptosis and inhibit cell proliferation through multiple mechanisms, including the modulation of critical signaling pathways involved in cell survival and programmed cell death. An additional notable advantage of Armillaria mellea is its non-toxicity, particularly in comparison to conventional chemotherapeutic agents, which frequently lead to adverse side effects. The present study aimed to investigate the anti-lung cancer effects of Armillaria mellea mycelium aqueous (AMMA) extract in the A549 cell line. MTT assay, Hoechst 33342, Fluo-4-AM staining, DCFH-DA staining and ELISA were utilized to evaluate changes in cell viability, nuclear apoptosis, Ca2+ concentration, intracellular reactive oxygen species (ROS) concentration, lactate dehydrogenase (LDH), malondialdehyde (MDA), catalase (CAT), superoxide dismutase (SOD), and the protein expression levels of Caspase-3/-8/-9. The results demonstrated that AMMA effectively reduced the survival rate of A549 cells, promoted nuclear apoptosis, calcium ion, intracellular ROS level, LDH and MDA content, inhibited high levels of CAT and SOD, and activated the Caspase-3/-8/-9 apoptosis pathway, ultimately mediating the death of cancer cells. This study confirmed that AMMA has an effective anti-lung cancer activity via the Caspase-3/-8/-9 pathway. These data also clarify the potential of AMMA as a natural product based therapeutic agent for the treatment of non-small cell lung cancer.","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":"52 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147913386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-06-01DOI: 10.1134/s1062359024613132
Yi Jiang, Haoyang Zhou, Rong Cheng, Yanan Pu, Di Wang, Yangyang Jiao, Xinyu Li, Yan Chen
Cardiovascular diseases (CVDs) threaten the human health. Lots of researches suggest that ROS accumulates in cardiomyocytes in CVDs. Excessive mitochondrial autophagy caused by reactive oxygen species (ROS) impairs the function of cardiomyocytes. Here, we found that extracellular vesicles derived human umbilical cord blood plasma (UCB-EV) inhibited oxidative stress-induced mitophagy to promote cell survival. The viability of UCB-EV pre-protected H9C2 cardiomyocytes was maintained after H2O2 exposure. In addition, UCB-EV partially restored mitochondrial membrane potential and promoted mitochondrial biogenesis. Furthermore, PTEN-induced putative kinase 1 (PINK1), a mitochondrial serine/threonine protein kinase, was inhibited by UCB-EV during oxidative stress. We further demonstrate the role of UCB-EV in inhibiting mitophagy to make H9C2 cells survival by PINK1/Parkin pathway. Our findings elucidate the protective mechanism of UCB-EV against oxidative stress in H9C2 cells. These findings reveal the potential role of UCB-EV in alleviate oxidative stress in cardiomyocytes.
{"title":"Extracellular Vesicles Derived from Human Umbilical Cord Blood Plasma Protect H2O2-Induced Oxidative Damage by Inhibiting Mitophagy in H9C2 Cells","authors":"Yi Jiang, Haoyang Zhou, Rong Cheng, Yanan Pu, Di Wang, Yangyang Jiao, Xinyu Li, Yan Chen","doi":"10.1134/s1062359024613132","DOIUrl":"https://doi.org/10.1134/s1062359024613132","url":null,"abstract":"Cardiovascular diseases (CVDs) threaten the human health. Lots of researches suggest that ROS accumulates in cardiomyocytes in CVDs. Excessive mitochondrial autophagy caused by reactive oxygen species (ROS) impairs the function of cardiomyocytes. Here, we found that extracellular vesicles derived human umbilical cord blood plasma (UCB-EV) inhibited oxidative stress-induced mitophagy to promote cell survival. The viability of UCB-EV pre-protected H9C2 cardiomyocytes was maintained after H2O2 exposure. In addition, UCB-EV partially restored mitochondrial membrane potential and promoted mitochondrial biogenesis. Furthermore, PTEN-induced putative kinase 1 (PINK1), a mitochondrial serine/threonine protein kinase, was inhibited by UCB-EV during oxidative stress. We further demonstrate the role of UCB-EV in inhibiting mitophagy to make H9C2 cells survival by PINK1/Parkin pathway. Our findings elucidate the protective mechanism of UCB-EV against oxidative stress in H9C2 cells. These findings reveal the potential role of UCB-EV in alleviate oxidative stress in cardiomyocytes.","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":"52 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147902190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Non-small cell lung cancer (NSCLC) is one of the most lethal types of malignancies around the world. Previously, LINC00852 is a tumor-associated lncRNA which has been verified to participate in affecting the activity of NSCLC cells in our work. However, its underlying molecular mechanism still remains poorly documented. The aim of this work is to further explore the involved regulatory mechanism of LINC00852 on NSCLC cells. Three NSCLC cell lines including H1299, A549 and LTEP-a2 cells were selected. LINC00852 was knockdown through its specific silence molecular ASO. Cell autophagy was analyzed by transmission electron microscope observation, fluorescent adenovirus infection and Western blot assays, respectively. Cell activity was measured through proliferation and colony formation assays. Knockdown the expression of LINC00852 leads to the activation of cell autophagy in three NSCLC cells. Previously, LINC00852 has been proved to act as a ceRNA of miR-29a-3p which further targets LAMTOR1. In this work, miR-29a-3p overexpression or LAMTOR1 knockdown both significantly increased the number of autolysosomes and promoted autophagy flux in NSCLC cells. Strikingly, the proliferation and colony formation abilities of NSCLC cells could be inhibited after treatment of rapamycin, an autophagy activator of cells. The results have indicated that knockdown of LINC00852 could impair the activities of NSCLC cells by activating cell autophagy via the LINC00852/miR-29a-3p/LAMTOR1 axis, which provides scientific guidance for the regulatory mechanism of long non-coding RNA LINC00852 in NSCLC cells, and also lay a theoretical basis for the potential treatment approach of NSCLC.
{"title":"Knockdown of LINC00852 Impairs the In Vitro Activities of Multiple Non-Small Cell Lung Cancer Cells through Activating Cell Autophagy","authors":"Boxiong Wu, Haozhe Cheng, Xinran Li, Wenjing Zhang, Qiancheng Li, Shuai Hao","doi":"10.1134/s1062359024609753","DOIUrl":"https://doi.org/10.1134/s1062359024609753","url":null,"abstract":"Non-small cell lung cancer (NSCLC) is one of the most lethal types of malignancies around the world. Previously, LINC00852 is a tumor-associated lncRNA which has been verified to participate in affecting the activity of NSCLC cells in our work. However, its underlying molecular mechanism still remains poorly documented. The aim of this work is to further explore the involved regulatory mechanism of LINC00852 on NSCLC cells. Three NSCLC cell lines including H1299, A549 and LTEP-a2 cells were selected. LINC00852 was knockdown through its specific silence molecular ASO. Cell autophagy was analyzed by transmission electron microscope observation, fluorescent adenovirus infection and Western blot assays, respectively. Cell activity was measured through proliferation and colony formation assays. Knockdown the expression of LINC00852 leads to the activation of cell autophagy in three NSCLC cells. Previously, LINC00852 has been proved to act as a ceRNA of miR-29a-3p which further targets LAMTOR1. In this work, miR-29a-3p overexpression or LAMTOR1 knockdown both significantly increased the number of autolysosomes and promoted autophagy flux in NSCLC cells. Strikingly, the proliferation and colony formation abilities of NSCLC cells could be inhibited after treatment of rapamycin, an autophagy activator of cells. The results have indicated that knockdown of LINC00852 could impair the activities of NSCLC cells by activating cell autophagy via the LINC00852/miR-29a-3p/LAMTOR1 axis, which provides scientific guidance for the regulatory mechanism of long non-coding RNA LINC00852 in NSCLC cells, and also lay a theoretical basis for the potential treatment approach of NSCLC.","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":"52 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147881518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-06-01DOI: 10.1134/s1062359024610243
Jing Xu, Yong Ji, Lei Xian, Yawen Zhang, Mengru Ming, Han Wu, Zhang Qi, Mi Shen
Schwann cells (SCs) play a crucial role in the repair of peripheral nerves. This is due to their ability to proliferate, migrate, and provide trophic support to axon regrowth. During peripheral nerve injury, SCs de-differentiate and reprogram to gain the ability to repair nerves. Cysteine-rich 61 (Cyr61/CCN1) is a member of the CCN family of matrix cell proteins, and we have reported its function in SC proliferation and migration. This study found that Cyr61 protein is up-regulated after nerve injury, and Cyr61 secreted by SCs promotes motor neuron growth. Cyr61 could promote motor neuron growth through autocrine or paracrine mechanisms. Our study could contribute to understanding cellular and molecular mechanisms underlying SC’s support for neurons and may facilitate the regeneration of peripheral nerves after injury.
{"title":"Schwann Cell Secreted Cyr61 Promotes Motor Neuron Growth","authors":"Jing Xu, Yong Ji, Lei Xian, Yawen Zhang, Mengru Ming, Han Wu, Zhang Qi, Mi Shen","doi":"10.1134/s1062359024610243","DOIUrl":"https://doi.org/10.1134/s1062359024610243","url":null,"abstract":"Schwann cells (SCs) play a crucial role in the repair of peripheral nerves. This is due to their ability to proliferate, migrate, and provide trophic support to axon regrowth. During peripheral nerve injury, SCs de-differentiate and reprogram to gain the ability to repair nerves. Cysteine-rich 61 (Cyr61/CCN1) is a member of the CCN family of matrix cell proteins, and we have reported its function in SC proliferation and migration. This study found that Cyr61 protein is up-regulated after nerve injury, and Cyr61 secreted by SCs promotes motor neuron growth. Cyr61 could promote motor neuron growth through autocrine or paracrine mechanisms. Our study could contribute to understanding cellular and molecular mechanisms underlying SC’s support for neurons and may facilitate the regeneration of peripheral nerves after injury.","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":"52 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147915356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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