Biosensors and Bioelectronics: X最新文献_第10页

Current state of the art on implantable sensors for continuous post-EVAR surveillance of abdominal aortic aneurysms: A comprehensive review 腹主动脉瘤evar后持续监测植入式传感器的研究现状：综述

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-10-01 Epub Date: 2025-06-12 DOI: 10.1016/j.biosx.2025.100645

G. Saroglia , S. Marocco , S. Nicoloso , S. Diana , M. Tozzi , I. Stefanini

Post-endovascular aneurysm repair (EVAR) surveillance currently relies on routine imaging techniques such as computed tomography angiography and magnetic resonance angiography, which expose patients to radiation, nephrotoxic contrast agents, and place logistical burdens on healthcare systems. This review critically examines the potential of implantable sensors, particularly pressure-monitoring systems, to provide continuous, minimally invasive post-EVAR surveillance. We present an overview of the biological and biomechanical rationale for monitoring aneurysm sac pressurization, analyze existing sensor technologies including EndoSure® and ImPressure®, and discuss technical challenges such as wireless telemetry, biocompatibility, signal attenuation, and power management. Regulatory milestones and alignment with ESVS and SVS surveillance guidelines are reviewed, along with the current evidence gap between pressure trends and clinical outcomes such as reintervention or rupture. While technical feasibility has been demonstrated in controlled settings, no studies to date have shown that pressure monitoring improves long-term outcomes or guides decision-making independently of imaging. The limitations of pressure-based monitoring are discussed alongside emerging sensor designs that integrate pressure, morphological, and biochemical sensing. This review concludes that implantable pressure sensors remain investigational tools whose clinical adoption will depend on future trials demonstrating diagnostic accuracy, patient acceptability, cost-effectiveness, and outcome benefit.

血管内动脉瘤修复后（EVAR）监测目前依赖于常规成像技术，如计算机断层血管造影和磁共振血管造影，这使患者暴露于辐射、肾毒性造影剂，并给医疗系统带来后勤负担。这篇综述严格审查了植入式传感器的潜力，特别是压力监测系统，以提供持续的、微创的evar后监测。我们概述了监测动脉瘤囊加压的生物学和生物力学原理，分析了现有的传感器技术，包括EndoSure®和ImPressure®，并讨论了无线遥测、生物相容性、信号衰减和电源管理等技术挑战。审查了监管里程碑和与ESVS和SVS监测指南的一致性，以及当前压力趋势与临床结果（如再干预或破裂）之间的证据差距。虽然技术上的可行性已经在受控环境下得到了证明，但迄今为止还没有研究表明压力监测可以改善长期结果或独立于成像指导决策。本文讨论了基于压力的监测的局限性以及集成压力、形态和生化传感的新兴传感器设计。本综述的结论是，植入式压力传感器仍然是研究工具，其临床采用将取决于未来的试验，以证明诊断的准确性、患者的可接受性、成本效益和结局效益。

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引用次数: 0

A simple MOF-based nanosensor for bisphenol A detection: in vitro application in supernatant of human endothelial cell models 用于双酚A检测的简单mof纳米传感器：在人内皮细胞模型上清液中的体外应用

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-10-01 Epub Date: 2025-08-06 DOI: 10.1016/j.biosx.2025.100666

Lorena Garcia-Rodrigo , Cristina Tortolini , Araceli González-Cortés , Ilenia Ventura , Silvia Migliaccio , Elisabetta Ferretti , Riccarda Antiochia

This work describes a simple electrochemical nanosensor for bisphenol A (BPA) detection based on an iron metal organic framework (MIL-100(Fe)) immobilized onto a commercial multiwalled carbon nanotubes (MWCNTs)/gold nanoparticles (AuNPs) modified graphite screen printed electrode (GSPE). The electrochemical platform has been characterized by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and SEM-EDX analysis. The resulting MIL-100(Fe)/AuNPs_MWCNTs/GSPE platform exhibited competitive analytical performances, including a wide dynamic range (0.1–100 μM), and a low detection limit (20 nM). Additionally, the proposed sensor demonstrated excellent selectivity, enabling accurate BPA detection in the presence of potential interferences. Lastly, the sensor has been successfully tested for in vitro BPA detection in the supernatant samples of human endothelial cellular models, Human Umbilical Vein Endothelial Cells (HUVECs) and Human Aortic Endothelial Cells (HAECs), exposed to BPA. These findings highlight the potential of the proposed nanosensor for the detection of BPA in supernatant cell fluids of individuals exposed to BPA at different concentrations and incubation times.

本工作描述了一种用于双酚a （BPA）检测的简单电化学纳米传感器，该传感器基于铁金属有机框架（MIL-100(Fe)）固定在商业多壁碳纳米管(MWCNTs)/金纳米颗粒（AuNPs）修饰的石墨丝网印刷电极（GSPE）上。利用循环伏安法（CV）、电化学阻抗谱（EIS）和SEM-EDX分析对电化学平台进行了表征。MIL-100(Fe)/AuNPs_MWCNTs/GSPE平台具有较好的分析性能，包括宽动态范围（0.1-100 μM）和低检出限（20 nM）。此外，所提出的传感器具有优异的选择性，能够在存在潜在干扰的情况下准确检测BPA。最后，该传感器已成功地在暴露于BPA的人内皮细胞模型、人脐静脉内皮细胞（HUVECs）和人主动脉内皮细胞（HAECs）的上清样品中进行了体外BPA检测。这些发现强调了所提出的纳米传感器在不同浓度和孵育时间暴露于双酚a的个体上清细胞液中检测双酚a的潜力。

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引用次数: 0

A wireless reader for detecting frequency shift of passive sensors 一种用于检测无源传感器频移的无线读取器

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-10-01 Epub Date: 2025-08-05 DOI: 10.1016/j.biosx.2025.100668

Fariborz Mirlou , Emine Bardakci , Taher Abbasiasl , Levent Beker

A body area sensor network (bodyNET) consists of wearable and implantable sensors to monitor human physiological signals. While passive disposable, chip-free, and batteryless sensors have been widely studied, benchtop equipment is still required for data collection, limiting usage to laboratory settings. Efficient integration of passive wearable and implantable sensors with dedicated compact readout circuitry is crucial for enabling data acquisition outside controlled laboratory environments. Here, we present a miniaturized, flexible wireless reader (WiRe) designed for frequency-shifting passive sensors, which also incorporates power harvesting capability. WiRe is compatible with a wide variety of passive sensors, offering a versatile platform for diverse medical applications. We deployed WiRe across various passive sensor platforms to track frequency shifts associated with biological signals. Our results show that WiRe uniquely combines power harvesting with a flexible, portable readout interface, enabling effective and non-invasive detection of even minor frequency shifts in a frequency sweep range of 1–8 MHz with 100 kHz resolution for both in vitro and in vivo experiments.

人体区域传感器网络（bodyNET）由可穿戴和植入式传感器组成，用于监测人体生理信号。虽然无源一次性、无芯片和无电池传感器已经被广泛研究，但数据收集仍然需要台式设备，限制了实验室环境的使用。无源可穿戴和植入式传感器与专用的紧凑型读出电路的有效集成对于在受控实验室环境外实现数据采集至关重要。在这里，我们提出了一种小型化的，灵活的无线读取器（WiRe），设计用于移频无源传感器，它还包含了能量收集功能。WiRe与各种无源传感器兼容，为各种医疗应用提供了一个多功能平台。我们在各种无源传感器平台上部署了WiRe，以跟踪与生物信号相关的频率变化。我们的研究结果表明，WiRe独特地将能量收集与灵活的便携式读出接口相结合，能够在体外和体内实验中以100 kHz分辨率在1-8 MHz的频率扫描范围内有效且无创地检测即使是较小的频率偏移。

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引用次数: 0

Advanced analysis of fully-printed organic transistors platform for multi-ion detection in sweat 汗液中多离子检测全印刷有机晶体管平台的先进分析

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-10-01 Epub Date: 2025-07-25 DOI: 10.1016/j.biosx.2025.100661

Silvia Demuru , Jaemin Kim , Ilya Kiselev , Guillaume Joël Jeanneret-Grosjean , Brince Paul Kunnel , Min Liu , Xi Chen , Mathieu Saubade , Celine Lafaye , Shih-Chii Liu , Danick Briand

引用次数: 0

Computational modeling of the effects of biofouling and foreign body response on continuous glucose monitors 生物污垢和异物反应对连续血糖监测仪影响的计算模型

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-10-01 Epub Date: 2025-08-21 DOI: 10.1016/j.biosx.2025.100676

John R. Aggas

Biofouling and eventual foreign body response are critical issues limiting the long-term performance of implantable continuous glucose monitors (CGMs). The implant is subjected to reduced blood flow in situ governed by the inflammatory response, resulting in decreased access to glucose and a decrease in sensor sensitivity (drift). In this work, a computational model of an amperometric 2^nd generation enzymatic glucose sensor is examined that incorporates the time-dependent changes in the interstitial environment due to the foreign body response (FBR). Appropriate physics are applied with respect to glucose transport from tissue, temporal foreign body response, enzyme kinetics, and measured electrochemical current. The resultant sensor performance is modeled against publicly available clinical data over a 14-day wear time, using test cases of an idealized implant that is not subjected to FBR, fibrous encapsulation with neovascularization, and fibrous encapsulation without neovascularization. The resultant sensitivity, lag-time, and sensor performance are compared against the reference clinical data. Results demonstrate that in silico modeling has utility in predicting in vivo performance, offering a vital tool for sensor design and pre-clinical optimization.

生物污垢和最终的异物反应是限制植入式连续血糖监测仪（cgm）长期性能的关键问题。植入物受到炎症反应控制的原位血流量减少，导致葡萄糖接触减少和传感器灵敏度降低（漂移）。在这项工作中，研究了第二代安培型酶促葡萄糖传感器的计算模型，该模型包含了由于异物反应（FBR）引起的间质环境的时间依赖性变化。适当的物理应用于葡萄糖从组织运输，时间异物反应，酶动力学和测量的电化学电流。所得到的传感器性能是根据14天的公开临床数据进行建模的，使用了不受FBR影响的理想植入物、有新生血管形成的纤维包埋和没有新生血管形成的纤维包埋的测试案例。所得的灵敏度、滞后时间和传感器性能与参考临床数据进行比较。结果表明，硅模型在预测体内性能方面具有实用价值，为传感器设计和临床前优化提供了重要工具。

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引用次数: 0

Nanoparticle-enabled portable biosensors for early detection and monitoring of non-communicable diseases: A focus on diabetes, cardiovascular, and cancer diagnostics 用于非传染性疾病早期检测和监测的纳米颗粒便携式生物传感器：重点关注糖尿病、心血管和癌症诊断

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-10-01 Epub Date: 2025-08-24 DOI: 10.1016/j.biosx.2025.100675

Anjali Upadhaya , Joynath Pegu , Yengkhom Disco Singh , Senpon Ngomle

The increasing global prevalence of non-communicable diseases (NCDs) such as diabetes, cardiovascular disorders, and cancer poses a significant challenge to healthcare systems, particularly in low resource settings. Improving patient outcomes requires early and accurate diagnosis, but existing diagnostic approaches frequently depend on invasive procedures and centralized labs, which limits their timeliness and accessibility. This review emphasizes the increasing importance of the nanoparticle-based portable biosensors as a pioneering strategy for these diagnostic issues. These emerging biosensing platforms provide a great combination of miniaturization, sensitivity, and instant detection, which are especially promising in point-of-care and personalized healthcare. Great advances in biosensor platforms in terms of detection of biomarkers had been made by inclusion of nanomaterials in these devices, showing potential for non-invasive and rapid disease monitoring at low-cost impact. It provides a comprehensive understanding of how nanotechnology-driven biosensors are reshaping the future of diagnostics for chronic diseases. In order to demonstrate how these systems might facilitate early intervention, ongoing monitoring, and wider access to healthcare, this paper compiles current advancements. Therefore, portable biosensors that use nanotechnology can contribute to quicker, simpler, and more accessible illness diagnosis, which will improve patient care everywhere.

糖尿病、心血管疾病和癌症等全球非传染性疾病（NCDs）的日益流行对卫生保健系统构成了重大挑战，特别是在资源匮乏的环境中。改善患者预后需要早期和准确的诊断，但现有的诊断方法往往依赖于侵入性手术和集中实验室，这限制了其及时性和可及性。这篇综述强调了基于纳米粒子的便携式生物传感器作为这些诊断问题的先驱策略的重要性日益增加。这些新兴的生物传感平台提供了小型化、灵敏度和即时检测的良好组合，在护理点和个性化医疗保健中尤其有前景。通过在这些装置中加入纳米材料，生物传感器平台在检测生物标志物方面取得了巨大进展，显示出以低成本影响进行非侵入性和快速疾病监测的潜力。它提供了对纳米技术驱动的生物传感器如何重塑慢性病诊断未来的全面理解。为了展示这些系统如何促进早期干预、持续监测和更广泛地获得医疗保健，本文汇编了当前的进展。因此，使用纳米技术的便携式生物传感器有助于更快、更简单、更容易的疾病诊断，这将改善各地的病人护理。

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引用次数: 0

Electrochemical immunosensors targeting hormonal biomarkers for polycystic ovary syndrome: Recent advances and analytical bottlenecks 针对多囊卵巢综合征激素生物标志物的电化学免疫传感器：最新进展和分析瓶颈

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-10-01 Epub Date: 2025-07-05 DOI: 10.1016/j.biosx.2025.100657

Aniket Nandi , Samuel W. Gillespie , S. Nick Ice , Divyanshi Thakur , Yash Kumar Gaur , Kamal Singh , Kalicharan Sharma

Polycystic Ovary Syndrome (PCOS) is a multifactorial endocrine disorder affecting up to 13 % of women of reproductive age, with nearly 70 % remaining undiagnosed due to the limitations of conventional diagnostic methods. These traditional approaches, based on imaging, hormonal assays, and symptom evaluation, are often delayed, nonspecific, and unsuitable for large-scale screening. Recent advances in electrochemical biosensors targeting hormonal biomarkers such as Anti-Müllerian Hormone (AMH), Sex Hormone-Binding Globulin (SHBG), and Follicle-Stimulating Hormone (FSH) offer a promising alternative for rapid, sensitive, and cost-effective PCOS diagnosis. This review critically evaluates electrochemical biosensor technologies developed in last five years for the detection of PCOS, discussing material choices, electrode fabrication strategies, signal transduction techniques (EIS, DPV, CV), and their analytical performance. Key limitations in analytical design, clinical validation, and commercialization are identified, including challenges with multiplexed detection, stability in real biological matrices, and scalability. Finally, solution strategies and future research directions are proposed to bridge the gap between laboratory innovation and real-world point-of-care diagnostic tools for PCOS.

多囊卵巢综合征（PCOS）是一种多因素内分泌疾病，影响多达13%的育龄妇女，由于传统诊断方法的限制，近70%的妇女未被诊断出来。这些基于影像学、激素检测和症状评估的传统方法往往是延迟的、非特异性的，不适合大规模筛查。针对激素生物标志物的电化学生物传感器的最新进展，如抗勒氏激素（AMH），性激素结合球蛋白（SHBG）和促卵泡激素（FSH），为快速，敏感和经济的多囊卵巢综合征诊断提供了一个有希望的替代方法。本文综述了近五年来用于PCOS检测的电化学生物传感器技术，讨论了材料选择、电极制造策略、信号转导技术（EIS、DPV、CV）及其分析性能。分析设计、临床验证和商业化的关键限制被确定，包括多重检测的挑战、真实生物基质的稳定性和可扩展性。最后，提出了解决方案策略和未来的研究方向，以弥合实验室创新与现实世界的PCOS即时诊断工具之间的差距。

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引用次数: 0

MoS2-Si3N4-based SPR biosensor for the detection of malaria at different stages: A theoretical insight 基于mos2 - si3n4的SPR生物传感器在不同阶段检测疟疾：一个理论见解

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-10-01 Epub Date: 2025-06-25 DOI: 10.1016/j.biosx.2025.100655

Talia Tene , Marco Guevara , Isaías Caicedo , Jose Luis Granizo Jara , Myrian Borja , Lala Gahramanli , Cristian Vacacela Gomez , Stefano Bellucci

The accurate differentiation of malaria stages is essential for effective treatment and epidemiological control. This work presents a theoretical analysis of a multilayer surface plasmon resonance (SPR) biosensor for malaria stage detection based on refractive index variations of infected red blood cells. The sensor combines silver, silicon nitride (Si₃N₄), monolayer molybdenum disulfide (MoS₂), and thiol-functionalized single-stranded DNA (ssDNA) on a BK7 prism. Using the transfer matrix method (TMM), we evaluate the sensor performance across Ring, Trophozoite, and Schizont stages through sensitivity, full width at half maximum (FWHM), quality factor (QF), detection accuracy (DA), limit of detection (LoD), and comprehensive sensitivity factor (CSF). The optimized configuration achieves angular sensitivities of 318.2 (Ring), 268.9 (Trophozoite), and 244.8 (Schizont) °/RIU, demonstrating clear stage discrimination and competitive performance compared to reported multilayer SPR sensors. The layered architecture was designed with experimentally accessible materials and configurations, supporting future translation to stage-specific diagnostic platforms.

疟疾分期的准确区分对于有效治疗和流行病学控制至关重要。这项工作提出了一个多层表面等离子体共振（SPR）生物传感器疟疾阶段检测的理论分析，基于感染红细胞的折射率变化。该传感器在BK7棱镜上结合了银、氮化硅（Si3N4）、单层二硫化钼（MoS2）和硫醇功能化单链DNA （ssDNA）。利用传递矩阵法（TMM），我们通过灵敏度、半最大全宽度（FWHM）、质量因子（QF）、检测精度（DA）、检测限（LoD）和综合灵敏度因子（CSF）来评估环、Trophozoite和Schizont阶段的传感器性能。优化后的配置实现了318.2（环形）、268.9 （Trophozoite）和244.8 （Schizont）°/RIU的角灵敏度，与已有的多层SPR传感器相比，具有清晰的阶段识别和竞争力。分层架构采用实验可访问的材料和配置设计，支持未来转换为特定阶段的诊断平台。

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引用次数: 0

WGM Sensors for future diagnostics: small molecules to whole organisms 用于未来诊断的WGM传感器：从小分子到整个生物体

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-10-01 Epub Date: 2025-08-07 DOI: 10.1016/j.biosx.2025.100671

Wilhelm Eugen Andreas Krames , Defne Ilayda Dayi , Lars Kaiser , Oliver Riester , Michael Himmelhaus , René Csuk , Hans-Peter Deigner

In a world facing constant challenges from diseases and pathogens, rapid diagnosis is crucial. Whispering gallery mode (WGM) resonators present an advanced sensor technology suitable for the detection of various analytes. Like surface plasmon resonance (SPR), they offer highly sensitive and selective measurements, ideal for medical diagnostics. Traditional WGM resonators, however, are immobilized on surfaces near optical couplers, making their production complex and costly. Nevertheless, a more recent approach employs polystyrene microspheres localized to cavities in fluidic chips as resonators, simplifying preparation and functionalization and displaying enhanced performance. Here, we assess the applicability of those sensors to different analyte sizes, ranging from small molecules (<1000 Da) to whole organisms. By employing different detection strategies, we confirm the applicability of the WGM sensors for detecting a wide range of targets.

The study focuses on L. pneumophila, TEM-1 beta-lactamase, aztreonam, and meropenem as representatives of these three major groups. We show that the detection was successful for all chosen analytes, while small molecules required additional signal amplification through a sandwich approach. Results of the analysis of the protein measurements showed an LOD of 0.09 μM and an LOQ of 0.28 μM with a R² greater than 0.99. Additionally, a comparative measurement demonstrates that the WGM is more sensitive than the widely used gold standard SPR (LOD 0.34 μM; LOQ 1.12 μM). Taken together, our work demonstrates the applicability of the WGM sensors to pathogen detection, protein quantification or small molecule analysis with an improved sensitivity compared to current gold standard methods.

在一个不断面临疾病和病原体挑战的世界，快速诊断至关重要。窃窃廊模式（WGM）谐振器提供了一种先进的传感器技术，适用于各种分析物的检测。与表面等离子体共振（SPR）一样，它们提供了高度敏感和选择性的测量，是医学诊断的理想选择。然而，传统的WGM谐振器固定在光学耦合器附近的表面上，使其生产复杂且成本高昂。然而，最近的一种方法是将聚苯乙烯微球定位在流控芯片的空腔中作为谐振器，简化了制备和功能化，并显示出增强的性能。在这里，我们评估了这些传感器对不同分析物大小的适用性，从小分子（<1000 Da）到整个生物体。通过采用不同的检测策略，我们证实了WGM传感器对广泛目标检测的适用性。本研究以嗜肺乳杆菌、TEM-1 β -内酰胺酶、氨曲南和美罗培南为代表。我们表明，检测是成功的所有选定的分析物，而小分子需要额外的信号放大通过三明治的方法。分析结果显示，LOD为0.09 μM， LOQ为0.28 μM, R2 > 0.99。此外，对比测量表明，WGM比广泛使用的金标准SPR (LOD 0.34 μM；Loq为1.12 μm)。综上所述，我们的工作证明了WGM传感器在病原体检测、蛋白质定量或小分子分析方面的适用性，与目前的金标准方法相比，其灵敏度有所提高。

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引用次数: 0

Design and fabrication of smartphone based single paper lateral flow assay for early detection of PCOS biomarker 基于智能手机的PCOS生物标志物早期检测单纸侧流法的设计与制作

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-10-01 Epub Date: 2025-08-07 DOI: 10.1016/j.biosx.2025.100672

Aniket Nandi , Yash Kumar Gaur , Divyanshi Thakur , Ekta Daweshar , Kalicharan Sharma

Polycystic Ovary Syndrome (PCOS) is a multifactorial endocrine disorder with significant diagnostic challenges, especially in early stages. Current diagnostic methods are often complex, time-consuming, and dependent on laboratory-based infrastructure. This study presents the design and fabrication of a smartphone-assisted paper-based lateral flow assay (LFA) for the early detection of PCOS via the quantification of sex hormone-binding globulin (SHBG), a promising metabolic biomarker. Fluorescent nitrogen-doped carbon dots (CDs) were synthesized using a microwave-assisted method and conjugated with anti-SHBG antibodies via EDC/NHS chemistry. The LFA strips were constructed using Whatman filter paper, and hydrophobic barriers were drawn using permanent marker ink. A portable device equipped with a 365 nm UV LED and a smartphone camera enabled fluorescence detection. Recombinant SHBG antigen samples in phosphate-buffered saline (PBS) were tested across a concentration range of 80–4000 ng/mL. The system exhibited a strong linear response (R² = 0.9677) between SHBG concentration and fluorescence intensity, with a calculated limit of detection (LOD) and limit of quantification (LOQ) of 2.68 ng/mL and 19.93 ng/mL, respectively. The analytical range of 80–4000 ng/mL corresponds to 0.8–42.1 nmol/L, covers the clinical diagnostic threshold of SHBG (<36 nmol/L). These results demonstrate the feasibility of a low-cost, point-of-care diagnostic tool for early PCOS screening. Further validation with clinical samples is necessary for real-world deployment.

多囊卵巢综合征（PCOS）是一种多因素内分泌疾病，具有显著的诊断挑战，特别是在早期。目前的诊断方法往往复杂、耗时，并且依赖于实验室基础设施。本研究介绍了一种智能手机辅助的基于纸张的横向流动试验（LFA）的设计和制造，通过定量性激素结合球蛋白（SHBG），一种有前途的代谢生物标志物，用于PCOS的早期检测。采用微波辅助法制备了荧光氮掺杂碳点（CDs），并通过EDC/NHS化学与抗shbg抗体偶联。使用Whatman滤纸构建LFA条带，并使用永久标记墨水绘制疏水屏障。配备365纳米紫外LED和智能手机摄像头的便携式设备可以进行荧光检测。重组SHBG抗原样品在磷酸盐缓冲盐水（PBS）中测试，浓度范围为80-4000 ng/mL。该体系SHBG浓度与荧光强度呈良好的线性关系（R2 = 0.9677），计算出的检测限（LOD）和定量限（LOQ）分别为2.68 ng/mL和19.93 ng/mL。80 ~ 4000 ng/mL的分析范围对应0.8 ~ 42.1 nmol/L，达到SHBG的临床诊断阈值（36 nmol/L）。这些结果证明了一种低成本、即时诊断的多囊卵巢综合征早期筛查工具的可行性。在实际应用中，需要对临床样本进行进一步验证。

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引用次数: 0