Biosensors and Bioelectronics: X最新文献_第7页

Green nanotechnology for implantable biosensors: Biocompatibility and functional integration in medical applications 可植入生物传感器的绿色纳米技术：医学应用中的生物相容性和功能集成

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-12-01 Epub Date: 2025-09-03 DOI: 10.1016/j.biosx.2025.100678

Amran Hossain , Mehedi Hasan Manik , Saifuddin Rakib , Naym Mahmud , Safiullah Khan , Zahid Ahsan , Md Safiul Islam , Nayem Hossain , Mosst Asma Akter

Green nanotechnology is increasingly leveraged to create biocompatible, environmentally friendly implanted biosensors that transform medical diagnostics without the ecological costs associated with conventional fabrication. Utilizing in-situ phytochemicals or microbial enzymes in plant extract, microbe, and biopolymer synthesis methods enables environmentally responsible nanoparticle synthesis of Graphene, Carbon Nanotubes (CNTs), Gold Nanoparticles (AuNPs), Silver Nanoparticles (AgNPs) and Quantum Dots(QDs) with greater cell viability and colloidal stability compared to those synthesized using the citrate reduction method. The functional integration of green-synthesized nanomaterials into biosensors enables nanomaterials to perform precise detection of biomarkers, such as glucose, lactate, and proteins, with high sensitivity, specificity, and signal transduction, for point-of-care applications and personalized medicine. Convergence of Internet of Things (IoT) integration in intelligent sensing networks that bridge biomedical diagnostics and environmental parameter monitoring, safety for chronic disease management, while minimizing contact, enhances the reliability of data and minimizes energy usage. Regulatory hurdles and critical challenges in translating from in vitro to in vivo applications, including surgical implantation risks, calibration drift, and chronic biocompatibility issues. Biodegradable electronics, AI-assisted analytics, and automated stimuli-responsive nanomaterials that adjust to physiological changes are highlighted as future directions. Bioresorbable sensors and self-healing polymers are examples of innovations that highlight the move toward patient-centered, sustainable healthcare. Green nanotechnology opens the door to implanted biosensors that balance environmental responsibility with state-of-the-art medical innovation by linking the fields of material science, bioengineering, and clinical practice. To overcome current obstacles and realize the full potential of implanted biosensors in precision medicine, this study emphasizes the need to develop green approaches.

绿色纳米技术越来越多地用于制造生物相容的、环境友好的植入式生物传感器，从而改变医疗诊断，而不需要传统制造带来的生态成本。与使用柠檬酸还原法合成的纳米颗粒相比，利用植物提取物、微生物和生物聚合物中的原位植物化学物质或微生物酶，可以合成对环境负责的纳米颗粒，包括石墨烯、碳纳米管（CNTs）、金纳米颗粒（AuNPs）、银纳米颗粒（AgNPs）和量子点（QDs），具有更高的细胞活力和胶体稳定性。绿色合成纳米材料与生物传感器的功能集成使纳米材料能够精确检测生物标志物，如葡萄糖、乳酸和蛋白质，具有高灵敏度、特异性和信号转导，适用于护理点应用和个性化医疗。物联网（IoT）集成在智能传感网络中的融合，在生物医学诊断和环境参数监测之间架起桥梁，为慢性疾病管理提供安全保障，同时最大限度地减少接触，提高数据可靠性并最大限度地减少能源消耗。从体外到体内应用的监管障碍和关键挑战，包括手术植入风险、校准漂移和慢性生物相容性问题。生物可降解电子、人工智能辅助分析和自动刺激响应纳米材料是未来的发展方向。生物可吸收传感器和自修复聚合物是创新的例子，突出了以患者为中心的可持续医疗保健的发展。绿色纳米技术打开了植入生物传感器的大门，通过连接材料科学、生物工程和临床实践领域，平衡环境责任和最先进的医疗创新。为了克服目前的障碍，实现植入生物传感器在精准医学中的全部潜力，本研究强调需要开发绿色方法。

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引用次数: 0

PNA-based biosensors for portable electrochemical diagnostics: Integration strategies and probe design 基于pna的便携式电化学诊断生物传感器：集成策略和探针设计

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-12-01 Epub Date: 2025-10-20 DOI: 10.1016/j.biosx.2025.100702

Younghwan Kim , Swomitra Kumar Mohanty

Portable and mobile biosensors are widely applied in point-of-care (POC) diagnostics due to their rapid response and suitability for decentralized testing. While DNA-based probes have been commonly used in electrochemical biosensors owing to their label-free operation, low cost, and sensitivity, their susceptibility to enzymatic degradation and low thermal stability has limited their broader utility. As an alternative, peptide nucleic acid (PNA) probes have gained increasing attention due to their synthetic backbone, which is based on an electrically neutral N-(2-aminoethyl)glycine (AEG) structure. This neutrality confers high resistance to nucleases and polymerases, while enabling stronger and more stable hybridization with negatively charged DNA targets. PNA probes also maintain structural integrity under low ionic strength conditions, improving signal stability compared to conventional DNA probes. These advantages have led to a growing body of research exploring PNA-based biosensors for portable applications. This mini-review surveys recent developments in this area, focusing on the physicochemical properties of PNA and their implications for biosensor design. Future directions for PNA biosensor development are also discussed. Representative studies on electrode architectures and detection strategies are highlighted, demonstrating the potential of PNA-based systems to support next-generation portable diagnostics.

便携式和移动生物传感器因其快速反应和适合分散检测而广泛应用于即时诊断（POC）。虽然基于dna的探针由于其无标记操作、低成本和灵敏度而被广泛用于电化学生物传感器，但它们对酶降解的敏感性和低热稳定性限制了它们的广泛应用。作为一种替代方法，肽核酸（PNA）探针由于其基于电中性N-（2-氨基乙基）甘氨酸（AEG）结构的合成骨架而受到越来越多的关注。这种中性赋予了对核酸酶和聚合酶的高抗性，同时使与带负电荷的DNA目标的杂交更强、更稳定。与传统DNA探针相比，PNA探针在低离子强度条件下也能保持结构完整性，提高信号稳定性。这些优点已经导致越来越多的研究机构探索pna为基础的便携式应用的生物传感器。本文综述了这一领域的最新进展，重点介绍了PNA的物理化学性质及其对生物传感器设计的影响。展望了PNA生物传感器的发展方向。重点介绍了电极结构和检测策略的代表性研究，展示了基于pna的系统支持下一代便携式诊断的潜力。

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引用次数: 0

Smart underwear: A novel wearable for long-term monitoring of gut microbial gas production via flatus 智能内衣：一种新颖的可穿戴设备，用于通过肠胃胀气长期监测肠道微生物气体的产生

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-12-01 Epub Date: 2025-10-10 DOI: 10.1016/j.biosx.2025.100699

Santiago Botasini , David Zhan , Norman Fischer , Charlotte T. Ravel , Ashley Tien , Maggie R. Grant , Glory Minabou Ndjite , Ty Sopko , Holly Childs , Maryann Greenfield , Christina X. Qian , Kara E. Gardiner , Nayantara M. Anders , Tasnim F. Ullah , Leah T. Redmond , Delaina A. Callaway , Eliya M. Behailu , Grace M. Sarkar , Nakati C. Sany , Margaret Slavin , Brantley Hall

The temporal dynamics of gut microbial metabolism, including how quickly and variably microbes respond to dietary changes, remain largely invisible to current research methods. While measuring metabolic outputs like short-chain fatty acids (SCFAs) in stool or blood provide insights into microbial metabolism, sampling frequency is severely limited, preventing observation of the temporal dynamics of how gut microbes respond to dietary inputs and environmental changes on an hour-by-hour basis. Hydrogen gas, produced by gut microbial metabolism, represents an underutilized biomarker for continuous monitoring of microbial metabolic activity. Following its production, hydrogen is expelled through two major routes: breath and flatus, with flatus containing dramatically higher hydrogen concentrations compared to breath, making it an ideal target for sensitive detection. Currently, no tools exist for continuous, non-invasive monitoring of hydrogen in flatus to track changes in gut microbial metabolism. To address this gap, we developed the Smart Underwear, the first wearable device that continuously measures hydrogen gas expelled in flatus, providing unprecedented temporal resolution of gut microbial activity. In a week-long User Experience Study, the Smart Underwear successfully tracked hydrogen in flatus with participants reporting high comfort and compliance. In a second study, the Smart Underwear accurately detected increased hydrogen production following inulin ingestion with 94.7 % sensitivity in 38 healthy participants, demonstrating its capability to track diet-induced changes in microbial metabolism. This novel platform enables longitudinal studies of microbiome function, capturing both inter-individual differences and intra-individual temporal dynamics, while opening new avenues for investigating diet-microbiome interactions, circadian patterns, and the functional consequences of changes in microbial composition.

肠道微生物代谢的时间动态，包括微生物对饮食变化的反应速度和变化程度，目前的研究方法在很大程度上仍然是不可见的。虽然测量粪便或血液中的短链脂肪酸（SCFAs）等代谢输出可以深入了解微生物代谢，但采样频率受到严重限制，无法观察肠道微生物如何对饮食输入和环境变化做出反应的时间动态。由肠道微生物代谢产生的氢气是一种未被充分利用的生物标志物，可用于持续监测微生物代谢活动。氢气产生后，通过两种主要途径排出：呼吸和放屁，与呼吸相比，放屁含有更高的氢气浓度，使其成为敏感检测的理想目标。目前，还没有工具可以连续、无创地监测肠胃胀气中的氢气，以跟踪肠道微生物代谢的变化。为了解决这个问题，我们开发了智能内衣，这是第一个可穿戴设备，可以连续测量肠胃胀气排出的氢气，提供前所未有的肠道微生物活动的时间分辨率。在一项为期一周的用户体验研究中，智能内衣成功地跟踪了放屁中的氢，参与者报告了高舒适度和依从性。在第二项研究中，在38名健康参与者中，智能内衣准确地检测到菊粉摄入后产生的氢气增加，灵敏度为94.7%，证明了其追踪饮食引起的微生物代谢变化的能力。这个新颖的平台能够对微生物组功能进行纵向研究，捕捉个体间差异和个体内时间动态，同时为研究饮食-微生物组相互作用、昼夜节律模式和微生物组成变化的功能后果开辟了新的途径。

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引用次数: 0

Corrigendum to “Flexible gold nanoparticle SERS tape for rapid, label-free and ultrasensitive detection and differentiation of Shiga toxin variants” [Biosens. Bioelectron. X 27 (2025) 100696] “用于快速、无标记和超灵敏检测和分化志贺毒素变体的柔性金纳米粒子SERS胶带”的勘误表[Biosens]。Bioelectron。x27 (2025) 100696]

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-12-01 Epub Date: 2025-10-17 DOI: 10.1016/j.biosx.2025.100704

A. D'Avino , A. Milano , V. Marchesano , B. Guilcapi , D. Sagnelli , M. Rippa , L. Zhou , G. Rossi , L. Consagra , M. Brigotti , S. Morabito , L. Petti

引用次数: 0

Cas12b-assisted one-step dual-target CRISPR system for Mycobacterium tuberculosis detection cas12b辅助一步双靶点CRISPR系统检测结核分枝杆菌

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-12-01 Epub Date: 2025-09-24 DOI: 10.1016/j.biosx.2025.100692

Xike Zhou , Xuchun Han , Lifang Liu , Xiaoping Wang , Junjie Lu , Long Qiu , Tao Wu , Hao Pei , Guping Zhao , Jin Wang

Tuberculosis (TB) remains a persistent public health threat, while the efficiency of traditional diagnostic methods is restricted. To address this, we developed a CRISPR-Cas12b-based one-step dual-target detection system (TB-QUICKv2) for sensitive and specific detection of the Mycobacterium tuberculosis complex (MTBC). Specifically, Cas12b and a molecular beacon (MB) probe were used to detect the target MTBC IS6110 sequence and an internal control (IC), the Bacillus spizizenii 16S sequence, in one reaction system, respectively. The optimal reaction temperature for TB-QUICKv2 was 57 °C and the detection procedure can be completed within 25 min. TB-QUICKv2 is highly specific (100 %) for MTBC with a limit of detection of 10.4 CFU per milliliter at a 95 % confidence interval. TB-QUICKv2 was further validated with clinical sputum samples and the results showed remarkably higher sensitivity (41/74) than the traditional culture method (37/74) and acid-fast bacillus (AFB) testing (33/74). Therefore, this study demonstrates that TB-QUICKv2 is a promising method for the rapid and accurate detection of MTBC and other infectious pathogens in the future.

结核病仍然是一个持续存在的公共卫生威胁，而传统诊断方法的效率受到限制。为了解决这个问题，我们开发了一种基于crispr - cas12b的一步双靶点检测系统（TB-QUICKv2），用于敏感和特异性检测结核分枝杆菌复合体（MTBC）。具体而言，Cas12b和分子信标（MB）探针分别在一个反应体系中检测目标MTBC IS6110序列和内部对照（IC） spizizenii 16S序列。TB-QUICKv2的最佳反应温度为57℃，可在25 min内完成检测。TB-QUICKv2对MTBC具有高度特异性（100%），在95%的置信区间内检测限为每毫升10.4 CFU。TB-QUICKv2在临床痰液样本中进一步验证，结果显示灵敏度（41/74）显著高于传统培养法（37/74）和抗酸杆菌（AFB）检测法（33/74）。因此，本研究表明，TB-QUICKv2是未来快速准确检测MTBC和其他感染性病原体的一种有前景的方法。

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引用次数: 0

Paper-based origami assisted and enhanced electroanalytical detection of β-Amyloid peptide in plasma samples 纸基折纸辅助和增强血浆样品中β-淀粉样肽的电分析检测

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-12-01 Epub Date: 2025-09-23 DOI: 10.1016/j.biosx.2025.100687

Antonella Miglione , Panagiota M. Kalligosfyri , Claudia Corbo , Donato Calabria , Mara Mirasoli , Marco Frasconi , Stefano Cinti

In this work, we demonstrate how the analytical performance towards β-amyloid (Aβ(1–42) detection can be easily improved by using a printed label-free electrochemical immunosensor coupled to an external paper-based origami. Coupling the immunosensor with a customized foldable origami allows for an increase of resulting sensitivity towards the detection of Aβ(1–42) in both standard and biological samples. The features of paper are fundamental for the analysis of Aβ(1–42), recognized as a central indicator of Alzheimer's pathology, considering the low levels in plasma associated with early-stage disease. The proposed strategy highlights up to a 5-fold signal enhancement in both buffer and plasma at low Aβ(1–42) concentrations, bringing the platform's performance toward clinical requirements. The immunoplatform has been systematically optimized through electrochemical characterization and parameter tuning, achieving limits of detection of 2.7 ng/mL in buffer and 4.7 ng/mL in undiluted plasma, with a linear range up to 1 μg/mL. The platform shows satisfactory repeatability (<10 %) and excellent selectivity in the presence of interfering plasma proteins. Overall, this work offers a promising foundation for the development of accessible and scalable tools for early Alzheimer's disease screening.

在这项工作中，我们展示了如何通过使用与外部纸基折纸耦合的印刷无标签电化学免疫传感器来轻松提高β-淀粉样蛋白（a β(1-42)）检测的分析性能。将免疫传感器与定制的可折叠折纸结合，可以提高对标准样品和生物样品中a β(1-42)检测的灵敏度。这篇论文的特点是分析a β(1-42)的基础，考虑到低水平的血浆与早期疾病相关，a β(1-42)被认为是阿尔茨海默病病理的中心指标。该策略强调在低浓度a β(1-42)下缓冲液和血浆中的信号增强高达5倍，使该平台的性能达到临床要求。通过电化学表征和参数调整对免疫平台进行了系统优化，在缓冲液中检测限为2.7 ng/mL，在未稀释血浆中检测限为4.7 ng/mL，线性范围可达1 μg/mL。该平台在干扰血浆蛋白存在下具有满意的重复性（< 10%）和优异的选择性。总的来说，这项工作为开发可获得和可扩展的早期阿尔茨海默病筛查工具提供了有希望的基础。

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引用次数: 0

Decoding point-of-care testing: A strategic tool for influenza and SARS-CoV-2 management in healthcare facilities 解码护理点检测：医疗机构中流感和SARS-CoV-2管理的战略工具

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-12-01 Epub Date: 2025-09-25 DOI: 10.1016/j.biosx.2025.100691

Caterina Ledda , Helena C. Maltezou , Gaetano Isola , Giuseppe Motta , Carmelina Daniela Anfuso , Claudio Costantino , Venerando Rapisarda

The emergence of SARS-CoV-2 and the seasonal burden of influenza have underscored the urgent need for rapid and decentralized diagnostic tools in healthcare settings. This review explores the role of Point-of-Care (POC) testing in controlling respiratory virus transmission, focusing on its technical foundations, clinical applications, and implementation challenges. A systematic literature screening was conducted across PubMed, Scopus, and Web of Science, prioritizing studies from 2020 onward, to examine the diagnostic performance, use cases, and operational constraints of rapid antigen and molecular POC platforms. Comparative analysis highlights substantial differences in analytical sensitivity, turnaround times, cost-effectiveness, and deployment logistics between isothermal amplification, RT-PCR-based, and immunochromatographic assays. Emerging technologies, including SERS-based biosensors and multiplex portable platforms, are also discussed for their potential to enhance specificity and scalability. A dedicated section addresses real-world barriers such as device cost, personnel training, sample throughput, and regulatory considerations. Figure-based summaries and performance tables are included to assist clinicians and policymakers in selecting appropriate tools. Finally, we propose a strategic framework for integrating POC diagnostics into infection control protocols in hospitals and occupational health services. The findings suggest that when appropriately selected and deployed, POC testing can significantly reduce transmission risk, optimize patient management, and minimize workforce disruption. These outcomes justify broader adoption of POC tools and continued investment in innovation and clinical validation.

SARS-CoV-2的出现和流感的季节性负担突出表明，迫切需要在卫生保健机构中使用快速和分散的诊断工具。本文综述了即时检测（POC）在控制呼吸道病毒传播中的作用，重点介绍了其技术基础、临床应用和实施挑战。在PubMed、Scopus和Web of Science上进行了系统的文献筛选，对2020年以后的研究进行了优先排序，以检查快速抗原和分子POC平台的诊断性能、用例和操作限制。对比分析强调了等温扩增、基于rt - pcr和免疫层析分析在分析灵敏度、周转时间、成本效益和部署后勤方面的实质性差异。新兴技术，包括基于sers的生物传感器和多路便携式平台，也因其增强特异性和可扩展性的潜力而被讨论。一个专门的部分解决了现实世界的障碍，如设备成本、人员培训、样品吞吐量和监管考虑。包括基于数字的摘要和绩效表，以帮助临床医生和决策者选择适当的工具。最后，我们提出了将POC诊断纳入医院和职业卫生服务感染控制协议的战略框架。研究结果表明，如果选择和部署得当，POC检测可以显著降低传播风险，优化患者管理，并最大限度地减少劳动力中断。这些结果证明了更广泛地采用POC工具并继续投资于创新和临床验证。

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引用次数: 0

Design and fabrication of an electrochemical nano-biosensor for the quick sensing of SARS CoV-2 用于SARS - CoV-2快速传感的电化学纳米生物传感器的设计与制造

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-12-01 Epub Date: 2025-09-30 DOI: 10.1016/j.biosx.2025.100695

Sonali Priyadarshini , Kanchan Karki , Sanjay Kumar , Lakshika Bhandari , Krishna Pal Singh , Narotam Sharma , Anuj Nehra

Graphene oxide (GO) emerged as a biosensing material due to its patternable features, electron transfer properties, high functionality, and greater surface area that enables sensitive point-of-use applications. This report outlines the design of an electrochemical biosensor composed of a polycarbonate track-etched (PCTE) nano-sieve platform with two silver electrodes, which can detect the surface glycoprotein of the severe acute respiratory syndrome coronavirus 2 (SARS CoV-2), specifically the Spike protein (S-protein), in a rapid and sensitive manner. To fabricate the GO material, we utilized the modified Hummers' method to convert graphite powder. In this study, we covalently immobilized SARS-CoV-2 specific antibodies onto an EDC-NHS functionalized nanosieve platform using two methods i.e., conventional or traditional method and protein-G mediated. The immobilization of these antibodies on the nanosieve platform was achieved through bio-linkage, resulting in specific interactions between the spike protein and the antibodies. These interactions led to a partial blockage of the nanosieve, this led to a considerable decrease in the ionic current for a voltage range of 1.0–2.0 V. The linear range was set between 3.6 mM and 3.6 aM. The detection limit was in nM in traditional method which was notably decreased to fM in protein-G mediated antibodies immobilization. Notably, when testing two non-specific proteins, bovine serum albumin (BSA) and influenza virus, under the same settings, there was no significant change in the current. This nano-biorecognition platform, or nanobiosensor, offers improved stability and higher sensitivity due to the integration of minor GO laminates.

氧化石墨烯（GO）因其图案化特性、电子转移特性、高功能性和更大的表面积而成为一种生物传感材料，可实现敏感的使用点应用。本文设计了一种由聚碳酸酯轨迹蚀刻（PCTE）纳米筛平台和两个银电极组成的电化学生物传感器，该传感器可以快速灵敏地检测严重急性呼吸综合征冠状病毒2 （SARS CoV-2）表面糖蛋白，特别是刺突蛋白（s蛋白）。为了制备氧化石墨烯材料，我们利用改进的Hummers方法对石墨粉进行转化。在本研究中，我们采用常规或传统方法和蛋白g介导两种方法将SARS-CoV-2特异性抗体共价固定在EDC-NHS功能化纳米筛平台上。这些抗体通过生物连锁固定在纳米筛平台上，导致刺突蛋白与抗体之间的特异性相互作用。这些相互作用导致纳米筛的部分堵塞，从而导致1.0-2.0 V电压范围内离子电流的显著降低。线性范围设定在3.6 mM和3.6 aM之间。传统方法检出限为nM，蛋白g介导的抗体固定化法检出限明显降至fM。值得注意的是，当检测两种非特异性蛋白质，牛血清白蛋白（BSA）和流感病毒时，在相同的设置下，电流没有显着变化。这种纳米生物识别平台或纳米生物传感器，由于集成了少量氧化石墨烯层压板，提供了更好的稳定性和更高的灵敏度。

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引用次数: 0

Design of a highly sensitive SPR biosensor for rapid malaria detection based on zinc telluride and blue phosphorus/tungsten disulfide material 基于碲化锌和蓝磷/二硫化钨材料的高灵敏度SPR疟疾快速检测生物传感器设计

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-12-01 Epub Date: 2025-10-29 DOI: 10.1016/j.biosx.2025.100709

Md. Al Amin Islam Utshob, Maymona Binte Juwel, Rawfarin Sabiha, Aminur Rahman, Safayat-Al Imam, Khandakar Mohammad Ishtiak

Malaria continues to be a prominent global health issue, particularly in regions facing drug-resistant Plasmodium strains and climate-induced transmission expansion. To address the need for rapid and sensitive diagnostic devices, this research proposes an innovative surface plasmon resonance (SPR) biosensor design based on a BK7-ZnTe-Ag-Fe₂O₃-BlueP/WS₂ multilayer structure. To analyze the proposed SPR structure, both the finite element method (FEM) and the transfer matrix method (TMM) employed. The optimal architecture utilizes ZnTe (12 nm) for plasmonic coupling and light confinement, Ag (40 nm) for enhanced plasmonic sensitivity, Fe₂O₃ (3 nm) for interaction with the surface and stability and a BlueP/WS₂ monolayer for enhanced evanescent field and signal response. This proposed biosensor possesses maximum angular sensitivity of 444 (Degree/RIU), FWHM of 4.63 (Degree), figure of merit (FOM) of 95.80 and quality factor (QF) of 95.83 (1/RIU), with particularly good detection of the ring stage of malaria infection. Comparative studies with other sensor configurations reveal considerable enhancement in sensitivity and detection accuracy. High linearity of performance parameters with refractive index changes verifies the strength and reliability of the sensor for real-time malaria detection. The optimized SPR biosensor represents a low-cost, scalable platform for malaria detection at an early stage and can be extended to other biomedical applications, particularly in resource-limited settings.

疟疾仍然是一个突出的全球卫生问题，特别是在面临耐药疟原虫菌株和气候引起的传播扩大的区域。为了满足快速灵敏诊断设备的需求，本研究提出了一种基于BK7-ZnTe-Ag-Fe2O3-BlueP/WS2多层结构的创新表面等离子体共振（SPR）生物传感器设计。采用有限元法（FEM）和传递矩阵法（TMM）对SPR结构进行分析。最佳结构利用ZnTe （12 nm）用于等离子体耦合和光约束，Ag （40 nm）用于增强等离子体灵敏度，Fe2O3 （3 nm）用于与表面相互作用和稳定性，BlueP/WS2单层用于增强倏逝场和信号响应。该传感器的最大角灵敏度为444（度/RIU）， FWHM为4.63（度），优值图（FOM）为95.80，质量因子（QF）为95.83 (1/RIU)，对疟疾感染的环期检测效果特别好。与其他传感器配置的比较研究表明，在灵敏度和检测精度方面有相当大的提高。性能参数随折射率变化呈高度线性关系，验证了传感器实时检测疟疾的强度和可靠性。优化后的SPR生物传感器是一种低成本、可扩展的疟疾早期检测平台，可扩展到其他生物医学应用，特别是在资源有限的环境中。

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引用次数: 0

Feasibility study of a smart insole with triboelectric energy harvesters for early flatfoot detection 带摩擦电能量采集器的智能鞋垫早期平足检测的可行性研究

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-10-01 Epub Date: 2025-07-07 DOI: 10.1016/j.biosx.2025.100649

Mohammad Alghamaz, Leila Donyaparastlivari, Alwathiqbellah Ibrahim, Nelson Fumo

This study introduces a self-powered smart insole system designed for real-time monitoring of foot health, with a specific focus on detecting flatfoot conditions. The insole integrates multiple identical triboelectric energy harvesters strategically positioned to capture electrical signals generated from ground reaction forces during daily activities such as walking, jogging, and running. Proof-of-concept testing was conducted on a single participant under two conditions: a healthy foot and a simulated flatfoot created by reducing the medial arch height by approximately 70%. In the healthy foot trials, the system demonstrated consistent and reliable performance, with negligible electrical output from the medial arch sensor due to minimal ground contact in this region. In contrast, the simulated flatfoot condition produced a significant increase in voltage output from the medial arch sensor, successfully identifying the abnormal foot mechanics associated with arch collapse. Additionally, a neural network was implemented to classify healthy and flatfoot conditions from the collected data, achieving an accuracy of 86% and a precision of 96%, demonstrating the feasibility of machine learning integration for automated flatfoot detection. Overall, the findings validate the smart insole’s capability as a promising tool for continuous foot health monitoring, early diagnosis of flatfoot, and future applications in personalized rehabilitation and preventative care.

本研究介绍了一种自供电智能鞋垫系统，旨在实时监测足部健康，特别关注检测平足状况。鞋垫集成了多个相同的摩擦电能量收集器，策略性地定位于捕获日常活动（如散步，慢跑和跑步）中地面反作用力产生的电信号。在两种情况下对单个参与者进行了概念验证测试：健康足和通过将内侧足弓高度降低约70%而产生的模拟扁平足。在健康足部试验中，该系统表现出一致和可靠的性能，由于该区域最小的地面接触，内侧足弓传感器的电输出可以忽略不计。相比之下，在模拟的平足条件下，内侧足弓传感器输出的电压显著增加，成功地识别出与足弓塌陷相关的异常足部力学。此外，利用神经网络从收集的数据中对健康和平底足进行分类，准确率分别为86%和96%，证明了机器学习集成用于自动平底足检测的可行性。总的来说，研究结果验证了智能鞋垫作为一种有前途的工具的能力，可以持续监测足部健康，早期诊断扁平足，以及未来在个性化康复和预防性护理中的应用。

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