Journal of Functional Foods最新文献_第2页

Polysaccharides from perilla (Perilla frutescens L.) seed induce mitochondrial apoptosis in HCT-116 cells via BID - BAX axis 紫苏多糖通过BID - BAX轴诱导HCT-116细胞线粒体凋亡

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2026-03-01 Epub Date: 2026-02-14 DOI: 10.1016/j.jff.2026.107209

Yan-Ling Zhang , Qi-Cong Chen , Wei-Le Liu , Shou-Bin Huang , Yu-Tai Li , Wen Zhong , Qiang Liu , Huo-Ji Chen , Cui-Ping Jiang , Shao-Wei Zheng , Chun-Yan Shen

Two homogeneous polysaccharides, PFSP-1 and PFSP-3, were isolated from perilla (Perilla frutescens L.) seeds (PFSP) and exhibited significant structural differences. Molecular weights of PFSP-1 and PFSP-3 were 3.58 × 10⁴ Da and 1.04 × 10⁷ Da, respectively. Monosaccharide composition analysis indicated that PFSP-1 consisted of fucose, glucose, and galactose in a molar ratio of 1.36:1.94:1, whereas PFSP-3 was composed of fucose, xylose, glucose, and galactose at a ratio of 3.63:1.21:19.62:1. Morphological observations revealed that PFSP-1 displayed irregular flocculent formations with smooth surfaces, while PFSP-3 showed continuous sheet-like structures. A comparison of bioactivity revealed that PFSP-1, but not PFSP-3, significantly inhibited the proliferation of HCT-116 colon cancer cells. PFSP-1 potently induced apoptosis as evidenced by increased TUNEL positivity, caspase-9/3 activation, and mitochondrial membrane potential collapse. Transcriptomics and validation identified BID and BAX as key mediators. Further validation confirmed that PFSP-1 promoted BID expression, facilitated BAX activation, and triggered mitochondrial dysfunction, ultimately leading to the execution of apoptosis via the caspase-9/caspase-3 cascade. These findings collectively demonstrate that structural variations, particularly in monosaccharide composition and molecular architecture, critically govern the differential anti-colon cancer activity of perilla seed polysaccharides.

从紫苏（perilla frutescens L.）种子（PFSP）中分离得到两种均质多糖PFSP-1和PFSP-3，它们在结构上存在显著差异。PFSP-1和PFSP-3的分子量分别为3.58 × 104 Da和1.04 × 107 Da。单糖组成分析表明，PFSP-1由焦糖、葡萄糖和半乳糖组成，摩尔比为1.36:1.94:1；PFSP-3由焦糖、木糖、葡萄糖和半乳糖组成，摩尔比为3.63:1.21:19:62:1。形态学观察显示，PFSP-1呈不规则的絮状结构，表面光滑，而PFSP-3呈连续的片状结构。生物活性比较显示，PFSP-1显著抑制HCT-116结肠癌细胞的增殖，而PFSP-3不显著。PFSP-1可以通过TUNEL阳性、caspase-9/3激活和线粒体膜电位塌陷的增加来诱导细胞凋亡。转录组学和验证鉴定BID和BAX是关键的介质。进一步验证证实，PFSP-1促进BID表达，促进BAX激活，引发线粒体功能障碍，最终通过caspase-9/caspase-3级联导致细胞凋亡。这些发现共同表明，结构变化，特别是单糖组成和分子结构的变化，在很大程度上决定了紫苏籽多糖抗结肠癌活性的差异。

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引用次数: 0

Plant-based functional foods for healthy aging: current trends, bioactive compounds, and future perspectives 健康衰老的植物性功能食品：当前趋势、生物活性化合物和未来展望

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2026-03-01 Epub Date: 2026-02-03 DOI: 10.1016/j.jff.2026.107155

Mangalambika Balaji , Rahul Vashishth , Utpal Das

Plant-based functional foods are gaining recognition for promoting healthy aging, enhancing skin and cognitive health, and addressing modern dietary needs through bioactive compounds. Rich in antioxidants, polyphenols, and dietary fibers, these foods help prevent chronic diseases, improve digestive health, and support overall wellness. Consumer interest is driven by growing health awareness and personalized nutrition goals. Key plant-derived ingredients such as phenolic compounds, dietary fibers, plant proteins, phytosterols, and omega-3 fatty acids confer diverse functional benefits. Bioactive compounds with anti-aging potential such as carotenoids, polyphenols, and probiotics, have been shown to support skin integrity and cognitive function. However, challenges such as sensory acceptance, nutrient bioavailability, and regulatory barrier remain. This review examines current consumer trends, key bioactive ingredients, and technological advancements in plant-based functional foods. It emphasizes the need for continued research to enhance efficacy and acceptance, highlights their potential as essential contributors to sustainable health and future nutrition strategies.

植物性功能食品在促进健康衰老、增强皮肤和认知健康以及通过生物活性化合物满足现代饮食需求方面正获得认可。这些食物富含抗氧化剂、多酚和膳食纤维，有助于预防慢性疾病，改善消化系统健康，并支持整体健康。消费者的兴趣受到日益增长的健康意识和个性化营养目标的推动。关键的植物源性成分，如酚类化合物、膳食纤维、植物蛋白、植物甾醇和omega-3脂肪酸，具有多种功能益处。具有抗衰老潜力的生物活性化合物，如类胡萝卜素、多酚和益生菌，已被证明可以支持皮肤完整性和认知功能。然而，感官接受、营养物质生物利用度和监管障碍等挑战仍然存在。本文综述了目前植物性功能食品的消费趋势、主要生物活性成分和技术进展。它强调需要继续进行研究，以提高功效和认可度，强调它们作为可持续健康和未来营养战略的重要贡献者的潜力。

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引用次数: 0

Research progress on self-assembled quercetin hydrogels: Preparation methods, properties, and their application for tissue engineering 自组装槲皮素水凝胶的研究进展：制备方法、性质及其在组织工程中的应用

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2026-03-01 Epub Date: 2026-02-03 DOI: 10.1016/j.jff.2026.107184

Hongkun Xue , Shiman Zhang , Xumeng Wang , Jiacheng Yang , Chuanqiang Liu , Jiaqi Tan

Tissue engineering, which can artificially construct functional tissues or organs to repair or replace tissue defects caused by diseases, traumas, or aging, has become a current research hotspot. Nevertheless, current traditional therapeutic methods in tissue engineering are confronted with numerous challenges, including limited sources, poor integration of grafts, and high immune rejection. Hence, there is an urgent need to research a new type of material to address these limitations. Self-assembled quercetin hydrogels (SAQHs), with their unique structural advantages and biological functions, have shown promising application prospects in various fields of tissue engineering, including bone, cartilage, and skin. The paper initially outlines the preparation methods and reviews the self-assembly strategies of SAQHs. Moreover, this article systematically reviews the regulatory rules of influencing factors on the self-assembly process and the final structure. Finally, this review also comprehensively summarizes the properties of SAQHs and their applications in bone, cartilage, and skin tissue engineering. The review breaks through the single-function limitation of traditional tissue engineering materials. Additionally, this paper focuses on the novel SAQHs with inherent biological activities and self-assembly properties, which integrates the core research results on their preparation, self-assembly strategies, regulatory rules, and multi-tissue engineering applications. This fills the current gap in the research on the correlation between self-assembly strategies and influencing factors of SAQHs. Furthermore, this article systematically clarifies the mechanisms and applicable conditions of different self-assembly strategies, and summarizes the application potential and optimization directions of SAQHs in various tissue engineering scenarios. The findings can provide important guidance for the application of SAQHs in the field of tissue engineering.

组织工程是通过人工构建具有功能的组织或器官来修复或替代因疾病、创伤或衰老引起的组织缺陷，已成为当前的研究热点。然而，目前组织工程中的传统治疗方法面临着许多挑战，包括来源有限、移植物整合不良、免疫排斥高。因此，迫切需要研究一种新型材料来解决这些限制。自组装槲皮素水凝胶（SAQHs）以其独特的结构优势和生物学功能，在骨、软骨、皮肤等组织工程领域有着广阔的应用前景。本文首先概述了SAQHs的制备方法，并对其自组装策略进行了综述。此外，本文还系统地回顾了影响因素对自组装过程和最终结构的调节规律。最后，综述了SAQHs的性质及其在骨、软骨和皮肤组织工程中的应用。突破了传统组织工程材料单一功能的局限。此外，本文还重点介绍了具有固有生物活性和自组装特性的新型SAQHs，整合了其制备、自组装策略、调控规则和多组织工程应用等方面的核心研究成果。这填补了目前自组装策略与影响因素相关性研究的空白。在此基础上，系统阐述了不同自组装策略的作用机制和适用条件，总结了SAQHs在各种组织工程场景下的应用潜力和优化方向。研究结果可为SAQHs在组织工程领域的应用提供重要指导。

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引用次数: 0

Novel human milk-derived peptide β-casein 65 repairs intestinal injury via modulating gut microbiota 新型人乳衍生肽β-酪蛋白65通过调节肠道微生物群修复肠道损伤

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2026-03-01 Epub Date: 2026-02-10 DOI: 10.1016/j.jff.2026.107203

Min Zhang , Liping Xu , Fan Zhang , Juyi Zhao , Yanjie Chen , Xiaotong Chen , Xiangyun Yan , Xiaohui Chen , Shushu Li , Jun Zhang , Shuping Han

Necrotizing enterocolitis (NEC) remains a leading cause of morbidity and mortality in preterm infants, and effective pharmacologic options are scarce. Studies have demonstrated that human milk peptides can improve NEC, β-casein 65 is a novel peptide derived from preterm human milk, yet its specific function and mechanism remain unclear. Here we evaluated the therapeutic potential role of β-casein 65 in a mouse model of NEC. β-casein 65 improved survival and mitigated abdominal distension and hematochezia in experimental NEC models. Concomitantly, β-casein 65 significantly reduced pro-inflammatory cytokines (TNF-α and IL-6 by approximately 56% and 71%, respectively) and increased intestinal barrier proteins compared with the NEC group. Using 16S rRNA sequencing and untargeted metabolomics, β-casein 65 altered gut microbiota profiling by lowering pathogenic Proteobacteria and promoting beneficial bacteria like Bifidobacterium and Akkermansia. Metabolomics analysis showed notable changes in immune-regulating metabolites, especially enriched in the IgA immune network. This finding not only provides a mechanistic understanding but also highlights β-casein 65 as a promising candidate for the development of preventative therapeutics for this devastating neonatal disease.

坏死性小肠结肠炎（NEC）仍然是早产婴儿发病率和死亡率的主要原因，有效的药物选择很少。研究表明，人乳肽可以改善NEC， β-酪蛋白65是一种从早产儿母乳中提取的新型肽，但其具体功能和机制尚不清楚。我们在小鼠NEC模型中评估了β-酪蛋白65的潜在治疗作用。β-酪蛋白65提高了实验性NEC模型的存活率，减轻了腹胀和便血。同时，与NEC组相比，β-酪蛋白65显著降低了促炎细胞因子（TNF-α和IL-6，分别降低了约56%和71%），并增加了肠道屏障蛋白。利用16S rRNA测序和非靶向代谢组学，β-酪蛋白65通过降低致病性变形杆菌和促进双歧杆菌和Akkermansia等有益细菌来改变肠道微生物群谱。代谢组学分析显示，免疫调节代谢物发生了显著变化，特别是在IgA免疫网络中富集。这一发现不仅提供了一种机制的理解，而且突出了β-酪蛋白65作为一种有希望的候选药物，用于开发这种毁灭性的新生儿疾病的预防性治疗。

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引用次数: 0

Effects of crude polysaccharides from Coix seeds fermented by Limosilactobacillus reuteri on hyperglycemia and gut microbiota in type 2 diabetic mice 罗伊氏乳酸杆菌发酵薏苡仁粗多糖对2型糖尿病小鼠高血糖和肠道菌群的影响

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2026-03-01 Epub Date: 2026-02-13 DOI: 10.1016/j.jff.2026.107192

Jingjing Wang , Zhoujie Yang , Huaiqiang Zhang , Qin Pan , Hui Shi , Bing Lin , Liyan Zhang , Ying Zhou , Tingting Feng

This study aimed to investigate the hypoglycemic effects of polysaccharides (FCSP) from coix seeds fermented by Limosilactobacillus reuteri. The extraction process of FCSP was optimized using Response Surface Methodology, and evaluated the hypoglycemic effects of FCSP both in vivo and in vitro. After process optimization, the extraction rate of FCSP was 8.74%. In vitro studies demonstrated that FCSP reduced oxidative stress levels in IR-HepG2 cells, significantly enhanced the activities of hexokinase (HK) and pyruvate kinase (PK), and promoted glucose consumption and glycogen synthesis. In T2DM mice, FCSP decreased glycated serum protein levels and improved glucose tolerance. The levels of blood lipids and oxidative stress were also significantly ameliorated. Furthermore, FCSP significantly reduced the Firmicutes/Bacteroidetes ratio and enhanced the abundance of beneficial microbiota, including Lactococcus, Akkermansia, Lactobacillus, Bifidobacterium, Weissella and Adlercreutzia in the mouse gut. Overall, these findings provide clear scientific evidence for the hypoglycemic potential of FCSP.

本研究旨在探讨罗伊氏乳酸杆菌发酵薏苡仁多糖的降血糖作用。采用响应面法对fsp的提取工艺进行优化，并对fsp的体内外降糖作用进行评价。工艺优化后，fsp的提取率为8.74%。体外研究表明，FCSP可降低IR-HepG2细胞氧化应激水平，显著提高己糖激酶（HK）和丙酮酸激酶（PK）活性，促进葡萄糖消耗和糖原合成。在T2DM小鼠中，FCSP降低了糖化血清蛋白水平，改善了葡萄糖耐量。血脂和氧化应激水平也明显改善。此外，FCSP显著降低了小鼠肠道中厚壁菌门/拟杆菌门的比例，提高了有益菌群的丰度，包括乳球菌、Akkermansia、乳杆菌、双歧杆菌、Weissella和阿德勒克氏菌。总之，这些发现为FCSP的降糖潜力提供了明确的科学证据。

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引用次数: 0

Synergistic effect of amyloglucosidase and resveratrol on reducing in vitro starch digestibility 淀粉糖苷酶和白藜芦醇对降低体外淀粉消化率的协同作用

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2026-03-01 Epub Date: 2026-02-05 DOI: 10.1016/j.jff.2026.107187

Sunita Karki , Nicola Gasparre , Thomas Netticadan , Cristina M. Rosell

Bread is a major dietary carbohydrate source, but its rapid starch digestion promotes a high glycemic index. Resveratrol (RSV), a plant polyphenol, and amyloglucosidase (AMG) can modify starch structure which could potentially alter the starch digestibility. This study examined whether the individual and combined use of RSV and AMG could lower starch digestibility while preserving bread quality. The pasting and hydrolysis behavior of white and whole-wheat flours enriched with RSV, AMG, or their combination were analyzed by Rapid Visco Analyzer and modeled with Box–Lucas kinetics. Breads were evaluated for texture, color, RSV recovery, and in-vitro starch digestibility. In white flour, AMG reduced peak viscosity, whereas RSV mitigated this effect and increased setback, indicating enhanced retrogradation. The combined RSV-AMG treatment yielded the lowest hydrolysis rate (k = 0.56 min⁻¹), suggesting a denser starch–polyphenol network that restricted enzymatic access. Whole-wheat flours, bran and fiber led to different effects, with RSV increasing starch retrogradation during cooling. The combination of the treatment led to softer white crumbs and reduced the RSV induced firmness of whole-wheat crumbs. In both breads, RSV shifted starch fractions from rapid digestible starch to slowly digestible starch in both matrices, slowing the in vitro starch hydrolysis. Overall, RSV and AMG acted synergistically to modulate starch chain length and matrix structure, reducing starch digestibility without compromising technological performance. These findings highlight a feasible strategy to design lower-glycemic breads through enzyme–polyphenol co-modulation of starch retrogradation and digestibility.

面包是主要的膳食碳水化合物来源，但其快速的淀粉消化促进了高血糖指数。植物多酚白藜芦醇（RSV）和淀粉葡糖苷酶（AMG）可以改变淀粉结构，从而可能改变淀粉的消化率。本研究考察了单独或联合使用RSV和AMG是否能在保持面包品质的同时降低淀粉消化率。采用快速粘度分析仪（Rapid Visco Analyzer）和Box-Lucas动力学模型分析了添加RSV、AMG或两者组合的白面粉和全麦面粉的糊化和水解行为。对面包的质地、颜色、RSV回收率和体外淀粉消化率进行了评估。在白面粉中，AMG降低了峰值粘度，而RSV减轻了这种影响并增加了挫折，表明增强了退化。RSV-AMG联合处理的水解率最低（k = 0.56 min−1），表明淀粉-多酚网络更致密，限制了酶的进入。全麦面粉、麸皮和纤维的影响不同，RSV在冷却过程中增加了淀粉的降解。这两种处理的组合使白面包屑变软，并降低了RSV诱导的全麦面包屑的硬度。在这两种面包中，RSV将淀粉组分从快速消化淀粉转移到缓慢消化淀粉，减缓了淀粉的体外水解。总的来说，RSV和AMG协同作用，调节淀粉链长度和基质结构，在不影响工艺性能的情况下降低淀粉消化率。这些发现强调了通过酶-多酚共同调节淀粉降解和消化率来设计低血糖面包的可行策略。

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引用次数: 0

Dietary succinoglycan riclin ameliorates spontaneous gut inflammation by restoring immune homeostasis in Period2-deficient mice 饲粮琥珀聚糖蓖泥素可通过恢复免疫稳态来改善自发性肠道炎症

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2026-03-01 Epub Date: 2026-02-10 DOI: 10.1016/j.jff.2026.107198

Junhao Liu , Xiaodong Xu , Sijia Sun , Tiantian Zhou , Yimeng Cai , Zi Wang , Zhao Ding

Dietary polysaccharides are promising candidates for managing inflammatory bowel disease (IBD). However, their efficacy is typically assessed in acute, chemically-induced models that poorly reflect human disease. We therefore evaluated the succinoglycan riclin in a novel spontaneous IBD model, Period2-deficient (Per2^−/−) mice. Following a 4-month dietary intervention, riclin significantly ameliorated spontaneous intestinal inflammation. Treatment reduced inflammatory immune cell infiltration, suppressed oxidative stress, and enhanced antioxidant capacity. Riclin also restored intestinal barrier integrity by upregulating tight junction proteins and rescued the gut microbiota dysbiosis induced by Period2 deficiency. These findings demonstrate that riclin's benefits are associated with a multi-targeted mechanism that includes remodeling of the gut microbiome, reinforcement of the antioxidant system, and restoration of immune and barrier homeostasis. This study positions the dietary succinoglycan riclin as a potent prebiotic and functional food ingredient for microbiota-directed therapy of chronic gut inflammation.

膳食多糖是治疗炎症性肠病（IBD）的有希望的候选者。然而，它们的功效通常是在急性、化学诱导的模型中评估的，这些模型不能很好地反映人类疾病。因此，我们在一种新的自发性IBD模型，Per2 - / -缺乏小鼠中评估了琥珀聚糖赤霉素。经过4个月的饮食干预，蓖麻素显著改善了自发性肠道炎症。治疗减少炎症免疫细胞浸润，抑制氧化应激，增强抗氧化能力。Riclin还可以通过上调紧密连接蛋白来恢复肠道屏障的完整性，挽救由缺钙引起的肠道菌群失调。这些发现表明，蓖麻素的益处与多靶点机制有关，包括肠道微生物群的重塑、抗氧化系统的增强、免疫和屏障稳态的恢复。本研究将膳食琥珀聚糖蓖麻素定位为一种有效的益生元和功能性食品成分，用于微生物导向的慢性肠道炎症治疗。

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引用次数: 0

The MHLWAAK peptide derived from C-phycocyanin ameliorated ulcerative colitis by regulating the PPAR pathway in vivo and in vitro 从c -藻蓝蛋白衍生的MHLWAAK肽通过调节PPAR通路在体内和体外改善溃疡性结肠炎

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2026-03-01 Epub Date: 2026-02-15 DOI: 10.1016/j.jff.2026.107199

Fenghua Xu , Yan Zheng , Yun Zhang , Kechun Liu , Shanshan Zhang , Bing Li , Guohai Su

Ulcerative colitis (UC) is a chronic, relapsing inflammatory disease of the colon. Compared with current therapeutic drugs, bioactive peptides are safer and more promising for UC prevention and treatment. Our previous studies demonstrated that the MHLWAAK peptide derived from C-phycocyanin exhibited excellent antioxidant and anti-inflammatory effects. However, its potential therapeutic effects on UC remain unexplored. In the present study, we aimed to evaluate the therapeutic potency of MHLWAAK in UC and its underlying mechanism using in vivo and in vitro experiments. The in vivo results showed that MHLWAAK effectively alleviated the inflammatory responses and intestinal tissue damage in the zebrafish model with UC induced by dextran sulfate sodium. An RNA-seq analysis revealed that MHLWAAK regulated the peroxisome proliferator-activated receptor (PPAR) pathway at the transcriptional level. The qRT-PCR results consistently indicated that lipid-related genes were upregulated after peptide administration. Additionally, in vitro studies demonstrated that MHLWAAK exerted potent anti-inflammatory effects on lipopolysaccharide-induced inflammatory responses by suppressing the expression of inflammatory genes and proteins. Additionally, western blotting revealed that PPARγ activation inhibited the NF-κB signaling pathways at the protein level after MHLWAAK administration. In summary, this study highlighted the anti-inflammatory activities of MHLWAAK in vitro and in vivo. MHLWAAK peptide development represents a novel and promising strategy for UC prevention and treatment.

溃疡性结肠炎（UC）是一种慢性、复发性的结肠炎症性疾病。与目前的治疗药物相比，生物活性肽在UC的预防和治疗中更安全，更有前景。我们前期的研究表明，由c -藻蓝蛋白衍生的MHLWAAK肽具有良好的抗氧化和抗炎作用。然而，其对UC的潜在治疗作用仍未被探索。在本研究中，我们旨在通过体内和体外实验来评估MHLWAAK对UC的治疗作用及其潜在机制。体内实验结果显示，MHLWAAK可有效缓解葡聚糖硫酸钠诱导的UC斑马鱼模型的炎症反应和肠道组织损伤。RNA-seq分析显示MHLWAAK在转录水平上调控过氧化物酶体增殖物激活受体（PPAR）通路。qRT-PCR结果一致表明，给药后脂质相关基因上调。此外，体外研究表明，MHLWAAK通过抑制炎症基因和蛋白的表达，对脂多糖诱导的炎症反应具有有效的抗炎作用。此外，western blotting结果显示，MHLWAAK给药后，PPARγ活化在蛋白水平上抑制NF-κB信号通路。综上所述，本研究突出了MHLWAAK在体外和体内的抗炎活性。MHLWAAK肽的开发代表了UC预防和治疗的一种新的和有前途的策略。

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引用次数: 0

Antagonistic effects of sturgeon meat protein hydrolysate on alcohol-induced liver injury in mice and screening of peptides that activate alcohol dehydrogenase 鲟鱼肉蛋白水解物对小鼠酒精性肝损伤的拮抗作用及激活酒精脱氢酶肽的筛选

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2026-03-01 Epub Date: 2026-02-10 DOI: 10.1016/j.jff.2026.107188

Yizhou Fang , Lechen Zhang , Zhengmei Yin , Jianhua Liu , Guangrong Huang

This study aimed to prepare and screen alcohol dehydrogenase (ADH)-activating peptides from sturgeon meat. Only myofibrillar protein had the potential to generate ADH-activating peptides through trypsin hydrolysis, and in vivo experiments demonstrated that the 1–3 kDa peptide fraction from the hydrolysate could prevent alcohol-induced liver injury by restoring ADH activity, enhancing antioxidant capacity, and reducing pro-inflammatory factors, with a preventive effect of 0.45 g/kg equivalent to 0.30 g/kg of metadoxine. Subsequently, two potential ADH-activating peptides, DIDIRKDLYANN and GVPADNVKDVIIW, were further screened via simulation-based prediction of molecular properties from the 1–3 kDa fraction. Synthetic validation not only confirmed the effectiveness of the screening but also revealed that the ADH activation ability of DIDIRKDLYANN even surpassed that of metadoxine at high concentrations, coupled with favorable solubility and non-allergenic properties. In conclusion, both the 1–3 kDa peptide fraction and DIDIRKDLYANN demonstrated significant industrial potential for development as anti-alcohol biopharmaceuticals or liver-protective functional foods.

本研究旨在从鲟鱼肉中制备和筛选乙醇脱氢酶（ADH）激活肽。只有肌纤维蛋白有可能通过胰蛋白酶水解产生ADH激活肽，体内实验表明，从该水解产物中提取的1-3 kDa肽片段可以通过恢复ADH活性、增强抗氧化能力、降低促炎因子来预防酒精性肝损伤，其预防作用为0.45 g/kg，相当于0.30 g/kg的美他多辛。随后，通过模拟预测1-3 kDa片段的分子特性，进一步筛选了两种潜在的adh激活肽，DIDIRKDLYANN和gvpadnvkdviw。合成验证不仅证实了筛选的有效性，还揭示了DIDIRKDLYANN在高浓度下的ADH激活能力甚至超过了metadoxine，并且具有良好的溶解度和非致敏性。综上所述，1-3 kDa肽段和DIDIRKDLYANN均具有作为抗酒精生物制药或保护肝脏功能食品开发的重要工业潜力。

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引用次数: 0

Extraction, characteristics and activities of polysaccharides from Lycium barbarum 枸杞多糖的提取、特性及活性研究

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2026-03-01 Epub Date: 2026-02-16 DOI: 10.1016/j.jff.2026.107191

Youjie Huang, Qiurui Hu, Gangliang Huang

Lycium barbarum has the characteristics of homology of food and medicine. Lycium barbarum polysaccharide (LBP) is a natural plant polysaccharide isolated from Lycium barbarum fruit and one of the key bioactive substances in Lycium barbarum. According to the latest scientific research, Lycium barbarum polysaccharide shows a wide range of biological effects, including anti-tumor, anti-oxidation, anti-inflammatory, regulating blood sugar, neuroprotection, reproductive protection, liver protection and so on, and it has good application potential and value in food, health care and medicine and other related fields. Ultrasonic extraction of Lycium barbarum polysaccharide is an efficient and energy-saving modern extraction technology. Its core principle is to use the cavitation effect, mechanical effect and thermal effect of ultrasonic to destroy plant cell walls and accelerate the dissolution of polysaccharide. Herein, the extraction technology, structural characteristics and activities of Lycium barbarum polysaccharide were systematically reviewed. The advantages and limitations of the existing technology were summarized in order to provide feasible reference for the standardization, standardization, scientific development and application of Lycium barbarum polysaccharide. However, we must overcome the bottlenecks in structural analysis, standardized production and clinical translation. Strengthening interdisciplinary and international cooperation will promote the transformation from basic research to industrial application, thus establishing a comprehensive and high-quality Lycium barbarum polysaccharide industrial chain and making contributions to human health.

枸杞具有食药同源的特点。枸杞多糖（LBP）是从枸杞果实中分离得到的天然植物多糖，是枸杞中重要的生物活性物质之一。根据最新的科学研究，枸杞多糖具有广泛的生物效应，包括抗肿瘤、抗氧化、抗炎、调节血糖、神经保护、生殖保护、肝脏保护等，在食品、保健和医药等相关领域具有良好的应用潜力和价值。超声波提取枸杞多糖是一种高效节能的现代提取技术。其核心原理是利用超声波的空化效应、机械效应和热效应破坏植物细胞壁，加速多糖的溶解。本文对枸杞多糖的提取工艺、结构特征及活性进行了系统的综述。总结现有技术的优势和局限性，为枸杞多糖的标准化、规范化、科学开发和应用提供可行性参考。然而，我们必须克服结构分析、标准化生产和临床翻译等瓶颈。加强跨学科和国际合作，促进基础研究向产业应用的转变，从而建立全面、优质的枸杞多糖产业链，为人类健康做出贡献。

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引用次数: 0