Journal of Functional Foods最新文献_第7页

Curcumin as a multi-target nutritional intervention alleviates exercise-induced injuries from overtraining: intervention efficacy and mechanism of action 姜黄素作为多靶点营养干预减轻过度训练所致运动损伤的干预效果及作用机制

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2026-02-01 Epub Date: 2026-01-23 DOI: 10.1016/j.jff.2026.107166

Senlin Lei , Shuyue Deng , Ruolan Shi , Yifu Ding , Xiaoan Chen , Xianhui Li

Curcumin (CUR), a natural polyphenol, exerts antioxidant, anti-inflammatory, and metabolic regulatory actions in exercise physiology and may help ameliorate overtraining-induced multi-organ dysfunction and delayed recovery states associated with overtraining syndrome (OTS). This review systematically summarizes the effects of CUR—across different doses, timing/duration regimens, and delivery systems—on exercise performance, functional recovery, and indices of muscle injury and multi-organ pathology in individuals with varying training status. Given that CUR is challenged by insufficient exposure due to low solubility, extensive first-pass metabolism, and rapid clearance, we introduce an “outcome-window–timing/duration–systemic exposure–outcome interpretation” framework to align systemic exposure with the temporal windows of recovery endpoints related to exercise-induced muscle damage (EIMD) (muscle function and muscle-damage–related indices). This framework is intended to improve the practical interpretability of dosing, supplementation timing, and delivery-system selection, while highlighting the decisive role of formulation type in exposure variability and cross-study comparability, as well as the moderating influence of training status on observable responses. Finally, future research directions were proposed, including optimized formulation and personalized supplementation strategies. Collectively, these insights support the development of multi-target, natural product–based interventions in sports medicine and encourage integrating phytochemicals into precision nutrition to promote recovery and optimize performance.

姜黄素（Curcumin， CUR）是一种天然多酚，在运动生理学中具有抗氧化、抗炎和代谢调节作用，可能有助于改善过度训练引起的多器官功能障碍和与过度训练综合征（OTS）相关的延迟恢复状态。这篇综述系统地总结了不同剂量、时间/持续时间方案和给药系统对不同训练状态个体的运动表现、功能恢复、肌肉损伤指标和多器官病理的影响。鉴于低溶解度、广泛的首过代谢和快速清除导致CUR暴露不足，我们引入了一个“结果窗口-时间/持续时间-系统暴露-结果解释”框架，将系统暴露与与运动诱导的肌肉损伤（EIMD）（肌肉功能和肌肉损伤相关指数）相关的恢复终点的时间窗口相结合。该框架旨在提高剂量、补充时间和给药系统选择的实际可解释性，同时强调配方类型在暴露变异性和交叉研究可比性中的决定性作用，以及训练状态对可观察反应的调节作用。最后，提出了今后的研究方向，包括优化配方和个性化补充策略。总的来说，这些见解支持运动医学中多目标、基于天然产品的干预措施的发展，并鼓励将植物化学物质整合到精确营养中，以促进恢复和优化表现。

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引用次数: 0

Activate the gut–adipose Axis: Forsythia tea alleviates diet-induced obesity via gut microbiota–dependent Browning of white adipose tissue 激活肠道脂肪轴：连翘茶通过肠道微生物依赖的白色脂肪组织褐变减轻饮食引起的肥胖

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2026-02-01 Epub Date: 2026-01-06 DOI: 10.1016/j.jff.2025.107149

Yue Lv , Jianchao Wang , Yue Li , Jiawei Wang , Yuekun Zhang , Huiwen Wu

This study investigated the mechanisms by which Forsythia tea (FT) alleviates diet-induced obesity via the gut-adipose axis. Following 8 weeks of high-fat diet (HFD) feeding to induce obesity in male C57BL/6 J mice, the HFD-fed mice were randomized into HFD and HFD + FT groups, with a normal diet (ND) group as control, for a subsequent 4-week intervention. The results demonstrated that FT significantly attenuated HFD-induced body weight gain and improved glucose tolerance and insulin sensitivity. Furthermore, FT effectively reversed dyslipidemia and suppressed systemic inflammation. Mechanistically, FT reshaped the gut microbiota, enhancing alpha diversity and enriching beneficial taxa. These microbial changes were associated with increased colonic short-chain fatty acid (SCFA) levels, particularly butyrate, and reinforced intestinal barrier integrity. Crucially, FT treatment markedly upregulated the expression of uncoupling protein 1 (UCP1) and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) in inguinal white adipose tissue (iWAT), indicating the activation of WAT browning. Correlation analyses strongly linked the enriched beneficial microbes to the improved metabolic phenotypes. In conclusion, our findings reveal that FT mitigates obesity and metabolic dysfunction by activating a microbiota-dependent gut-adipose axis that promotes WAT browning, supporting its potential as a functional beverage for obesity management.

本研究探讨连翘茶（FT）通过肠道脂肪轴缓解饮食性肥胖的机制。以高脂饮食（HFD）诱导雄性C57BL/6 J小鼠肥胖8周后，将HFD喂养的小鼠随机分为HFD组和HFD + FT组，以正常饮食（ND）组为对照，进行后续4周的干预。结果表明，FT可显著减弱hfd诱导的体重增加，改善葡萄糖耐量和胰岛素敏感性。此外，FT有效地逆转血脂异常和抑制全身炎症。从机制上讲，FT重塑了肠道微生物群，增强了α多样性并丰富了有益的分类群。这些微生物变化与结肠短链脂肪酸（SCFA）水平的增加（尤其是丁酸盐）和肠道屏障完整性的增强有关。重要的是，FT处理显著上调了解偶联蛋白1 （UCP1）和过氧化物酶体增殖物激活受体γ辅助激活因子1- α (PGC-1α)在腹沟白色脂肪组织（iWAT）中的表达，表明WAT褐变活化。相关分析表明，有益微生物的富集与代谢表型的改善密切相关。总之，我们的研究结果表明，FT通过激活微生物依赖的肠道脂肪轴来减轻肥胖和代谢功能障碍，该轴促进WAT褐变，支持其作为肥胖管理功能饮料的潜力。

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引用次数: 0

Metabolomic study on the inhibitory effect of soluble dietary fiber from foxtail millet on colorectal cancer 谷子可溶性膳食纤维对结肠癌抑制作用的代谢组学研究

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2026-02-01 Epub Date: 2026-01-24 DOI: 10.1016/j.jff.2026.107162

Jieya Yang , Ruipeng Yang , Ruijun Su , Lihua Wu , Wenjing Zhao , Shuhua Shan , Runzhi Ren , Jincheng Xu

Colorectal cancer (CRC) is a malignant tumor of the digestive tract, and previous studies demonstrated that soluble dietary fiber from foxtail millet (FMB-SDF) exhibits significant anti-colorectal cancer effects both in vivo and in vitro. However, the metabolic changes of FMB-SDF in colorectal cancer models remain incompletely understood. This study focused on FMB-SDF. Using AOM/DSS to induce CRC in C57BL/6 J mice, an in vivo colorectal cancer model was established. LC-MS untargeted metabolomics technology was used to detect fecal metabolites in each group of mice to find out the potential biomarkers of CRC, to screen the potential metabolic pathways of FMB-SDF to intervene in CRC, and then to explore the mechanism of action of the anti-cancer effect of FMB-SDF based on the metabolic pathways. This study found that FMB-SDF can regulate some microbial metabolites in the intestine, enhance oxidative stress in colon cancer cell line HCT116, inhibit the secretion of inflammatory factors, and exert anti colorectal cancer effects. From the perspective of sphingolipid metabolism, after treatment with Myriocin, the recovery effect of FMB-SDF on weight loss, colon shortening, and intestinal barrier damage in colon cancer mice decreased, confirming that the anti colon cancer effect of FMB-SDF is closely related to the regulation of sphingolipid metabolic pathway. This provides new insights into FMB-SDF as a functional food for the prevention and treatment of colorectal cancer.

结直肠癌（Colorectal cancer， CRC）是一种消化道恶性肿瘤，前期研究表明谷子可溶性膳食纤维（FMB-SDF）在体内和体外均具有显著的抗结直肠癌作用。然而，FMB-SDF在结直肠癌模型中的代谢变化仍不完全清楚。本研究的重点是FMB-SDF。采用AOM/DSS诱导C57BL/ 6j小鼠结直肠癌，建立体内结直肠癌模型。采用LC-MS非靶向代谢组学技术检测各组小鼠的粪便代谢物，寻找CRC的潜在生物标志物，筛选FMB-SDF干预CRC的潜在代谢途径，进而基于代谢途径探索FMB-SDF抗癌作用的作用机制。本研究发现FMB-SDF可调节肠道内部分微生物代谢物，增强结肠癌细胞系HCT116的氧化应激，抑制炎症因子的分泌，发挥抗结直肠癌的作用。从鞘脂代谢的角度来看，肉豆豆素治疗后，FMB-SDF对结肠癌小鼠体重减轻、结肠缩短、肠屏障损伤的恢复作用减弱，证实FMB-SDF的抗结肠癌作用与调节鞘脂代谢途径密切相关。这为FMB-SDF作为预防和治疗结直肠癌的功能性食品提供了新的见解。

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引用次数: 0

Fucoxanthin mitigates lipopolysaccharide-induced acute liver injury by inhibiting hepatocyte apoptosis and pyroptosis 岩藻黄素通过抑制肝细胞凋亡和焦亡来减轻脂多糖诱导的急性肝损伤

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2026-02-01 Epub Date: 2026-01-28 DOI: 10.1016/j.jff.2026.107180

Yu-Hong Yang , Qing-Yan Zou , Cheng-Yu Yang , Hui Li , Zi-Jian Wu , Shi-Xiang Wu , Yan Guan , Ya-Ru Li , Kazuo Miyashita , Chang-Sheng Zhao , Lei Du

Acute liver injury (ALI) induced by lipopolysaccharide (LPS) represents a critical clinical challenge due to its high mortality and limited therapeutic options. Fucoxanthin (Fx), a marine-derived xanthophyll carotenoid, exerts hepatoprotective potential owing to its strong anti-inflammatory and antioxidant properties. However, its protective role against LPS-induced ALI remains poorly understood. This study elucidated the protective effect and underlying mechanism of Fx against LPS-induced ALI in both a mouse model and a hepatocyte-macrophage co-culture system. Oral administration of 50 or 200 mg/kg body weight Fx once daily for 7 consecutive days notably attenuated LPS-induced increase in serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, and improved pathological changes in mouse livers. In the co-culture system of AML12 hepatocytes and RAW264.7 macrophages, Fx treatment dose-dependently alleviated LPS-induced damage to AML12 cells, as evidenced by reduced lactate dehydrogenase (LDH) release and maintained cell viability. Mechanistically, Fx significantly suppressed macrophage overactivation-mediated hepatic inflammatory responses and oxidative stress induced by LPS. Specifically, Fx downregulated pro-inflammatory molecules, such as tumor necrosis factor-alpha (TNF-α), interleukin-1beta (IL-1β), IL-6, IL-18, and inducible nitric oxide synthase (iNOS), as well as inhibited intracellular reactive oxygen species (ROS) generation. These effects collectively suppressed excessive hepatocyte apoptosis via blocking the intrinsic apoptotic signaling pathway and inhibited massive hepatocyte pyroptosis through inactivating the NOD-like receptor family pyrin domain containing 3 (NLRP3)/caspase-1/ gasdermin D (GSDMD)-mediated pyroptosis pathway. Collectively, these findings suggest that Fx may serve as a promising functional ingredient for preventing ALI.

脂多糖（LPS）引起的急性肝损伤（ALI）由于其高死亡率和有限的治疗选择而成为一个关键的临床挑战。岩藻黄素（Fx）是一种源自海洋的类黄素类胡萝卜素，由于其强大的抗炎和抗氧化特性，具有保护肝脏的潜力。然而，其对lps诱导的ALI的保护作用仍然知之甚少。本研究在小鼠模型和肝细胞-巨噬细胞共培养系统中阐明了Fx对lps诱导的ALI的保护作用及其机制。口服Fx 50或200 mg/kg体重，每天1次，连续7天，可显著降低lps诱导的血清谷丙转氨酶（ALT）和天冬氨酸转氨酶（AST）水平升高，改善小鼠肝脏病理变化。在AML12肝细胞和RAW264.7巨噬细胞共培养系统中，Fx剂量依赖性地减轻了lps诱导的AML12细胞损伤，表现为乳酸脱氢酶（LDH）释放减少，维持了细胞活力。机制上，Fx显著抑制巨噬细胞过度激活介导的肝脏炎症反应和LPS诱导的氧化应激。具体而言，Fx下调促炎分子，如肿瘤坏死因子-α (TNF-α)、白细胞介素-1β (IL-1β)、IL-6、IL-18和诱导型一氧化氮合酶（iNOS），并抑制细胞内活性氧（ROS）的产生。这些作用通过阻断固有的凋亡信号通路共同抑制过量的肝细胞凋亡，并通过失活nod样受体家族pyrin结构域3 (NLRP3)/caspase-1/ gasdermin D （GSDMD）介导的焦亡途径抑制大量肝细胞焦亡。总的来说，这些发现表明Fx可能是预防ALI的有希望的功能成分。

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引用次数: 0

Anticancer effects of Chlorella sorokiniana Shihira and R. W. Krauss extract via Bcl-2/Bax modulation and AMPK-α activation in hepatoma cells 小球藻Shihira和R. W. Krauss提取物通过Bcl-2/Bax调节和AMPK-α活化对肝癌细胞的抗癌作用

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2026-02-01 Epub Date: 2026-01-28 DOI: 10.1016/j.jff.2026.107172

Min Ho Han , Min Ho Kang , Youn Seon Hwang , Seung Won Nam , Chang Soo Lee , Jin Woo Kim

Research on selectively inducing apoptosis in cancer cells is ongoing, yet effective natural compounds with fewer side effects are still needed. This study investigated the anticancer potential of Chlorella sorokiniana extract (CSE) in hepatoma cells. CSE showed high TPC (4.49 mg GAE/g DM) and TFC (0.86 mg QE/g DM) with strong ROS scavenging activity (9.0–45.0%). LC–MS/MS analysis suggested lutein, liquiritigenin, isorhamnetin, kaempferol as key metabolites responsible for apoptotic effects. CSE suppressed Hep3B cell viability by 22.1% without cytotoxicity in HepG2 cells and induced apoptosis via upregulation of Bax, AMPK-α, and caspase-3 (28.9–83.5%) and downregulation of Bcl-2 and VEGF (33.0–43.9%). Notably, apoptosis was enhanced (25.7%) in p53-deficient Hep3B cells, implying heightened susceptibility to CSE. These findings suggest C. sorokiniana has the potential to serve as a natural compound capable of selectively inducing apoptosis in liver cancer cells, indicating its potential as a foundational material for drug development.

选择性诱导癌细胞凋亡的研究正在进行中，但仍然需要副作用小的有效天然化合物。研究了小球藻提取物（Chlorella sorokiniana extract， CSE）对肝癌细胞的抗癌作用。CSE具有较高的TPC （4.49 mg GAE/g DM）和TFC (0.86 mg QE/g DM)，具有较强的ROS清除活性（9.0% ~ 45.0%）。LC-MS /MS分析提示叶黄素、利尿素、异鼠李素、山奈酚是参与细胞凋亡的主要代谢物。CSE通过上调Bax、AMPK-α和caspase-3(28.9-83.5%)，下调Bcl-2和VEGF（33.0-43.9%）诱导HepG2细胞凋亡，对Hep3B细胞的活性有22.1%的抑制作用。值得注意的是，p53缺失的Hep3B细胞凋亡增加（25.7%），表明对CSE的易感性增加。这些发现表明sorokiniana具有作为一种能够选择性诱导肝癌细胞凋亡的天然化合物的潜力，表明其作为药物开发的基础材料的潜力。

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引用次数: 0

A synbiotic supplement reduces body fat and increases GLP-1 secretion in a pilot clinical study 合成补充剂减少体脂和增加GLP-1分泌在试点临床研究

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2026-02-01 Epub Date: 2026-01-20 DOI: 10.1016/j.jff.2026.107171

Chiao-Wei Lin , Hsin-Bai Zou , Rou-An Chen , Chi-Shan Li , Chia-Ying Lin , Cheng-Chih Hsu

Obesity is closely associated with gut microbiota and their metabolites. We developed a multi-component synbiotic supplement that includes lychee polyphenols, Bifidobacterium longum, Bifidobacterium animalis, methionine, and other supportive ingredients such as prebiotics and postbiotics. A 5-week pilot clinical trial was conducted to evaluate its effects on body composition, metabolic markers, gut microbiota-derived metabolites, and GLP-1 secretion. The results showed significant reductions in body weight, fat mass, visceral fat, HbA1c, insulin resistance, and blood lipids. Metabolite analysis revealed increases in short-chain fatty acids and tryptophan metabolites, including acetic acid, isobutyric acid, isovaleric acid, serotonin, and indolelactic acid. GLP-1 secretion increased in both humans and mice, with a notable rise in Akkermansia muciniphila observed in mice given the synbiotic. These findings suggest that this synbiotic supplementation could serve as a potential approach for improving metabolic health and reducing obesity.

肥胖与肠道菌群及其代谢产物密切相关。我们开发了一种多组分合成补充剂，包括荔枝多酚、长双歧杆菌、动物双歧杆菌、蛋氨酸和其他支持性成分，如益生元和益生后。一项为期5周的先导临床试验评估了其对身体成分、代谢标志物、肠道微生物衍生代谢物和GLP-1分泌的影响。结果显示体重、脂肪量、内脏脂肪、糖化血红蛋白、胰岛素抵抗和血脂显著降低。代谢物分析显示短链脂肪酸和色氨酸代谢物增加，包括乙酸、异丁酸、异戊酸、血清素和吲哚乳酸。人和小鼠的GLP-1分泌均增加，在给予合成物的小鼠中观察到嗜粘液阿克曼氏菌的显著增加。这些发现表明，这种合成补充剂可以作为一种改善代谢健康和减少肥胖的潜在方法。

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引用次数: 0

Heat-killed Bifidobacterium longum LB-P23 ameliorates metabolic dysfunction in high-fat and high-sucrose diet-fed mice through gut microbiota modulation 热灭活长双歧杆菌LB-P23通过调节肠道菌群改善高脂高糖饮食小鼠的代谢功能障碍

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2026-02-01 Epub Date: 2026-01-20 DOI: 10.1016/j.jff.2026.107160

Won Jun Kim , Mikyung Song , Hangsu Cho , Ahjin Kim , Jaeseok Shim , Kyoungsub Song

The gut microbiota plays a pivotal role in obesity and metabolic disorders. This study evaluated the anti-obesity effects of heat-killed Bifidobacterium longum LB-P23 in a high-fat, high-sucrose diet-induced obese mouse model. Mice received low (1 × 10⁸ TCC) or high (1 × 10⁹ TCC) doses of B. longum LB-P23 for 8 weeks. High-dose B. longum LB-P23 significantly reduced body weight gain, insulin resistance and hepatic steatosis, while enhancing thermogenesis, and gut barrier integrity by regulating genes related to insulin signaling, β-oxidation, thermogenesis, and tight junctions. It also alleviated diet-induced dysbiosis, reducing Parabacteroides, and Colidextribacter, while enriching Romboustia, whose abundance positively correlated with plasma IL-22, indicating activation of IL-22 signaling. In vitro, B. longum LB-P23 suppressed lipid accumulation in 3T3-L1 adipocytes. Collectively, heat-killed B. longum LB-P23 exerts multi-organ metabolic benefits through modulation of insulin signaling, thermogenesis, and gut–immune interactions, supporting its potential as a safe anti-obesity intervention.

肠道菌群在肥胖和代谢紊乱中起着关键作用。本研究在高脂肪、高糖饮食诱导的肥胖小鼠模型中评估了热灭活长双歧杆菌LB-P23的抗肥胖作用。小鼠接受低剂量（1 × 108 TCC）或高剂量（1 × 109 TCC）长梭菌LB-P23治疗8周。高剂量B. longum LB-P23可显著降低体重增加、胰岛素抵抗和肝脏脂肪变性，同时通过调节胰岛素信号、β-氧化、产热和紧密连接相关基因，增强产热和肠道屏障完整性。它还减轻了饮食引起的生态失调，减少了拟副杆菌和Colidextribacter，同时丰富了Romboustia，其丰度与血浆IL-22正相关，表明IL-22信号通路被激活。在体外实验中，长刺草LB-P23抑制3T3-L1脂肪细胞的脂质积累。综上所述，热杀伤长芽胞杆菌LB-P23通过调节胰岛素信号、产热和肠道免疫相互作用发挥多器官代谢益处，支持其作为安全抗肥胖干预的潜力。

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引用次数: 0

Dietary polyphenols as functional food bioactives: Nuclear factor erythroid 2-related factor 2 (Nrf2)-mediated antioxidant and immunomodulatory mechanisms in combating bacterial infections 膳食多酚作为功能性食品生物活性物质：核因子-红细胞2相关因子2 （Nrf2）介导的抗氧化和免疫调节机制在对抗细菌感染中的作用

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2026-02-01 Epub Date: 2026-01-18 DOI: 10.1016/j.jff.2026.107165

Nada Ahmed , Mohamed El-Fateh , Moussa S. Diarra , Xin Zhao

The rising threat of antimicrobial resistance (AMR) highlights the need for novel strategies to fight against microbial pathogens while strengthening host defenses. Dietary polyphenols abundant in fruits, vegetables, and food byproducts, could offer dual antimicrobial and host-protective effects. At the same time, nuclear factor erythroid 2-related factor 2 (Nrf2) activation emerges as one of key mechanisms that extend beyond antioxidant control to regulate cytokine production and innate immune signaling. This review discusses the underexplored role of polyphenol in activating Nrf2 as a signaling hub that coordinates host immune responses against bacterial infection. Despite bioavailability hurdles, polyphenols could offer a promise direction for functional foods-based strategies in infection control.

抗菌素耐药性（AMR）的威胁日益严重，这表明需要新的策略来对抗微生物病原体，同时加强宿主的防御。富含水果、蔬菜和食品副产品的膳食多酚具有抗菌和保护宿主的双重作用。与此同时，核因子红细胞2相关因子2 （Nrf2）的激活成为超越抗氧化控制，调节细胞因子产生和先天免疫信号传导的关键机制之一。这篇综述讨论了多酚在激活Nrf2作为协调宿主免疫反应对抗细菌感染的信号中枢中的作用。尽管存在生物利用度障碍，但多酚可以为基于功能食品的感染控制策略提供一个有希望的方向。

引用次数: 0

A natural sulfated polysaccharide of high molecular weight, a fucoidan isolated from Undaria pinnatifida, is incorporated into Peyer's patches of mice via M cells and induces innate immune responses 从裙带菜中分离出一种高分子量的天然硫酸酸化多糖岩藻聚糖，通过M细胞进入小鼠的Peyer's斑块，诱导先天免疫反应

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2026-02-01 Epub Date: 2026-01-12 DOI: 10.1016/j.jff.2025.107147

Kyoko Hayashi , Jung-Bum Lee , Kohei Sasaki , Satomi Asai

Fucoidans (FUs) are sulfated glycans primarily found in marine algae and exhibit various bioactivities. Mekabu FU, from the edible brown alga Undaria pinnatifida, enhances innate and adaptive immunity and exerts protective effects in virus-infected mice, although its mechanisms remain unclear. We aimed to investigate the fate of mekabu FU after oral administration in mice by monitoring the uptake of fluorescence-labeled FU (FL-FU) into Peyer's patches (PPs) through M cells in the intestine and conducting DNA microarray analysis of PPs. FL-FU was observed in M cells 30 min after oral administration in mice. DNA microarray analysis of gene expression in PPs revealed that FU administration altered the expression of genes involved in immune regulation. Orally administered FL-FU was not metabolized into small fragments upon excretion from the mouse intestine, suggesting that mekabu FU suppresses viral infections by modulating intestinal immunity after incorporation into PPs through M cells.

岩藻聚糖（FUs）是一种主要存在于海洋藻类中的磺化聚糖，具有多种生物活性。Mekabu FU来自可食用褐藻裙带藻，可增强病毒感染小鼠的先天和适应性免疫，并发挥保护作用，尽管其机制尚不清楚。我们的目的是通过监测荧光标记FU （FL-FU）通过肠道M细胞进入Peyer's patches (PPs)，并对PPs进行DNA芯片分析，研究口服给药后小鼠mekabu FU的命运。口服给药30 min后，小鼠M细胞中观察到FL-FU的变化。基因表达的DNA芯片分析显示，FU给药改变了参与免疫调节的基因的表达。口服给药的FL-FU在从小鼠肠道排出后不会代谢成小片段，这表明mekabu FU通过M细胞掺入PPs后通过调节肠道免疫来抑制病毒感染。

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引用次数: 0

Fractionation, characterization, and gastrointestinal stability of anticancer and antioxidant peptides from sea cucumber (Holothuria scabra) hydrolysates 海参（Holothuria scabra）水解物中抗癌和抗氧化肽的分离、表征和胃肠道稳定性

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2026-02-01 Epub Date: 2026-01-23 DOI: 10.1016/j.jff.2026.107173

Supansa Saiwong , Utoomporn Surayot , Narongchai Autsavapromporn , Charin Tachapan , Yuthana Phimolsiripol , Sang Guan You , Hui Hong , Sun Young Lim , Sutee Wangtueai

Sea cucumber hydrolysates with antioxidant and anticancer properties were prepared using papain hydrolysis. The protein hydrolysates were fractionated into peptide groups of >10 kDa (SCPH-I), 3–10 kDa (SCPH-II), 1–3 kDa (SCPH-III), and < 1 kDa (SCPH-IV) using ultrafiltration membranes. The antioxidant and anticancer properties of both crude and fractionated hydrolysates were evaluated. The SCPH-III fraction exhibited the most potent antioxidant properties, with scavenging activities against the 2,2-diphenyl-1-picryl hydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), hydrogen peroxide (H₂O₂), and hydroxyl (OH) radicals of 87.96%, 78.48%, 39.39%, and 80.29%, respectively. The antiproliferative effect of the four fractions in the HepG2 cell model showed no significant difference (p > 0.05). The SCPH-III fraction was selected for peptide characterization using LC-MS/MS with de novo sequencing. Eighteen peptides were identified, among which two peptides demonstrated potent antioxidant and anticancer potential: Thr-Ser-Asp-Gly-Gln-Asn-Tyr-Leu-Leu-Leu-Lys and Phe-His-Val-Asp-Glu-Leu-Lys. The SCPH-III fraction induced significant morphological changes and apoptosis in HepG2 cells, with 87.30% of cells undergoing apoptosis at a 5 mg/mL concentration. Furthermore, the SCPH-III fraction was relatively stable under in vitro gastrointestinal digestion, showing a slight decrease in DPPH and OH radical scavenging activities, whereas the ABTS radical scavenging activity and anticancer activity increased significantly (p < 0.05).

采用木瓜蛋白酶水解法制备了具有抗氧化和抗癌特性的海参水解物。用超滤膜将蛋白水解产物分离成10 kDa （SCPH-I）、3-10 kDa （SCPH-II）、1 - 3 kDa （SCPH-III）和1 kDa （SCPH-IV）的肽组。对粗水解产物和分馏水解产物的抗氧化和抗癌性能进行了评价。SCPH-III部位对2,2-二苯基-1-吡啶酰肼（DPPH）、2,2 ' -氮基-双（3-乙基苯并噻唑-6-磺酸）、过氧化氢（H2O2）和羟基（OH）自由基的清除率分别为87.96%、78.48%、39.39%和80.29%，具有较强的抗氧化活性。四组分在HepG2细胞模型中的抗增殖作用差异无统计学意义（p > 0.05）。选择SCPH-III部分，采用LC-MS/MS与从头测序进行肽段表征。共鉴定出18条多肽，其中2条多肽具有较强的抗氧化和抗癌作用：thr - ser - asp - gly - gln - asn - tyrl - leu - leu - lys和pheh - his - val - asp - glu - leu - lys。SCPH-III组分在HepG2细胞中引起了明显的形态学改变和凋亡，在5 mg/mL浓度下，87.30%的细胞发生凋亡。体外胃肠消化作用下，SCPH-III组分相对稳定，对DPPH和OH自由基的清除能力略有下降，而对ABTS自由基的清除能力和抗癌能力显著提高（p < 0.05）。

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