Journal of Agricultural and Food Chemistry最新文献_第7页

A Paeoniae RadixAlba Polysaccharide Fraction BP-1Restores Intestinal Barrier Function and Gut Microbiota–MetabolicHomeostasis in Collagen-Induced Arthritis Rats 白芍多糖bp -1对胶原诱导关节炎大鼠肠道屏障功能和肠道微生物代谢稳态的恢复作用

IF 6.1 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry

Pub Date : 2026-10-15 DOI: 10.1021/acs.jafc.6c06645

Yongchun Wang,Yongxi Wu,Yuhan Xie,Qiulu Zhao,Jianing Liu,Meihua Jiang,Yinlu Zhang,Songyang Xia,Wenshuo Zhang,Hao Yue,Zifeng Pi

Abstract Paeoniae Radix Alba (PRA) polysaccharides are promising food-derived bioactives, yet their gut-mediated effects on rheumatoid arthritis (RA) remain unclear. Here, BP-1 was purified, structurally characterized, and evaluated in collagen-induced arthritis (CIA) rats and lipopolysaccharide (LPS)-injured Caco-2 cells. BP-1 was a 14.124 kDa neutral polysaccharide mainly composed of glucose and mannose. NMR analysis identified →4)-α-D-Glcp-(1→, →4,6)-α-D-Glcp-(1→, α-T-D-Glcp-(1→, →4,6)-β-D-Manp-(1→, and α-T-D-Galp-(1→ residues. BP-1 alleviated arthritis severity, joint pathology, systemic inflammation, and colonic injury, while improving intestinal barrier integrity in vivo and in vitro. These effects were potentially associated with suppression of TLR4/MyD88/NF-κB signaling, restoration of immune homeostasis, and modulation of gut microbiota and fecal metabolites. These findings support BP-1 as a potential functional food ingredient for inflammation-associated intestinal and immune disorders.

摘要白芍（Paeoniae Radix Alba， PRA）多糖是一种很有前景的食源性生物活性物质，但其对类风湿性关节炎（RA）的肠道介导作用尚不清楚。在这里，BP-1被纯化、结构表征，并在胶原诱导关节炎（CIA）大鼠和脂多糖（LPS）损伤的Caco-2细胞中进行了评估。BP-1为14.124 kDa中性多糖，主要由葡萄糖和甘露糖组成。核磁共振分析确定→4)-α-D-Glcp -(1→→4,6)-α-D-Glcp -(1→α-T-D-Glcp -(1→→4,6)-β-D-Manp -(1→,α-T-D-Galp -(1→残留。BP-1减轻了关节炎的严重程度、关节病理、全身炎症和结肠损伤，同时提高了体内和体外肠道屏障的完整性。这些作用可能与TLR4/MyD88/NF-κB信号的抑制、免疫稳态的恢复以及肠道微生物群和粪便代谢物的调节有关。这些发现支持BP-1作为炎症相关肠道和免疫疾病的潜在功能性食品成分。

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引用次数: 0

The Rhizobial TypeIII Effector NopD Regulates the GmPP2C-ENOD93 Axisto Control Symbiotic Nodulation and Yieldin Soybean 根瘤菌iii型效应物NopD调控GmPP2C-ENOD93轴对称调控大豆共生结瘤和产量

IF 6.1 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry

Pub Date : 2026-10-15 DOI: 10.1021/acs.jafc.6c07326

Liang Mi,Liying Lu,Jiake Sun,Yansong Li,Chao Ma,Shengnan Ma,Mingliang Yang,Siyan Hong,Miao Wang,Lin Chen,Chunyan Liu,Dawei Xin,Qingshan Chen,Jinhui Wang

Abstract Type III effector proteins (T3Es) are pivotal orchestrators of host–microbe interactions and essential for establishing symbiosis, thereby influencing soybean growth and productivity through modulation of symbiotic nitrogen fixation (SNF). While the central role of the T3E NopD in soybean SNF is recognized, its mechanistic underpinnings have remained elusive. In this study, we determined that GmPP2C, whose expression is regulated by NopD, serves as a regulator of nodulation. Overexpression of GmPP2C suppressed infection-thread formation and markedly reduced nodule number, whereas CRISPR/Cas9-mediated disruption enhanced infection events and partially rescued nodulation defects of NopD deficiency. Transcriptome profiling and complementation assays indicate GmPP2C inhibits nodulation by repressing ENOD93. Loss of GmPP2C increased per-plant yield without altering plant architecture or seed quality. These findings delineate the NopD–GmPP2C–ENOD93 regulatory module governing soybean nodulation and pinpoint GmPP2C as a compelling target for molecular breeding to enhance nitrogen-fixation efficiency and bolster crop yields.

III型效应蛋白（Type III effector protein, T3Es）是宿主与微生物相互作用的关键协调者，是建立共生关系的必要条件，从而通过调节共生固氮（symbiotic nitrogen fixation， SNF）影响大豆的生长和产量。虽然T3E NopD在大豆SNF中的核心作用已被认识到，但其机制基础仍然难以捉摸。在这项研究中，我们确定GmPP2C的表达受NopD的调节，作为结瘤的调节剂。过表达GmPP2C抑制了感染线的形成，显著减少了结节数量，而CRISPR/ cas9介导的破坏增强了感染事件，部分挽救了NopD缺乏症的结节缺陷。转录组分析和互补分析表明，GmPP2C通过抑制ENOD93来抑制结瘤。损失GmPP2C可提高单株产量，但不会改变植株结构或种子质量。这些发现描述了控制大豆结瘤的NopD-GmPP2C-ENOD93调控模块，并指出GmPP2C是提高固氮效率和提高作物产量的分子育种的一个引人注目的目标。

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引用次数: 0

In Situ ActiveSite-Engineered Z-Scheme CMCN/Co3O4-ZIF-8Heterostructure for EfficientTetracycline Degradation CMCN/ co3o4 - zif -8异质结构对四环素的有效降解

IF 6.1 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry

Pub Date : 2026-10-15 DOI: 10.1021/acs.jafc.6c09760

Wen Hao,Xueyan Cui,Lin Cai,Yichuan Cao,Guozhen Fang,Shuo Wang

Abstract Antibiotic contamination poses environmental risks and requires efficient degradation technologies. Herein, a Z-scheme heterostructured photocatalyst (CMCN/Co3O4-ZIF-8) was constructed by thermally coupling precursor-modified graphitic carbon nitride (CMCN) with metal–organic framework (MOF) derivative (Co3O4-ZIF-8) for tetracycline degradation. Temperature-regulated pyrolysis of ZnCo-MOF afforded Co3O4-ZIF-8 with in situ-formed Co3O4 active sites and a porous framework. CMCN/Co3O4-ZIF-8 heterointerface promoted charge separation and migration via interfacial electric field and energy gradient, enabling Z-scheme charge-transfer pathway that retained highly reductive electrons on Co3O4-ZIF-8 and strongly oxidative holes on CMCN. The Co2+/Co3+ redox cycle in Co3O4-ZIF-8 further facilitated interfacial electron transfer and O2 activation. Superoxide radicals and oxidative holes participated in tetracycline degradation, achieving a degradation efficiency of 86.88%. LC-MS analysis revealed major tetracycline degradation pathways, and toxicity evaluation indicated reduced toxicity of most intermediates. This study guided catalyst design by integrating in situ active site formation and Z-scheme charge-transfer pathway for antibiotic removal.

抗生素污染具有环境风险，需要高效的降解技术。本文采用前驱体修饰的石墨氮化碳（CMCN）与金属有机骨架（MOF）衍生物（Co3O4-ZIF-8）热偶联的方法，构建了用于四环素降解的z型异质结构光催化剂（CMCN/Co3O4-ZIF-8）。ZnCo-MOF的温度调节热解使Co3O4- zif -8具有原位形成的Co3O4活性位点和多孔骨架。CMCN/Co3O4-ZIF-8异质界面通过界面电场和能量梯度促进电荷分离和迁移，实现了在Co3O4-ZIF-8上保留高还原电子和CMCN上保留强氧化空穴的Z-scheme电荷转移途径。Co3O4-ZIF-8中的Co2+/Co3+氧化还原循环进一步促进了界面电子转移和O2活化。超氧自由基和氧化空穴参与四环素的降解，降解效率为86.88%。LC-MS分析揭示了主要的四环素降解途径，毒性评价表明大多数中间体毒性降低。本研究通过整合原位活性位点形成和Z-scheme电荷转移途径来指导抗生素去除的催化剂设计。

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引用次数: 0

Metabolic EngineeringStrategies for Rare d -Allulose Production from d -Glucose– d -Xylose Mixtures Using Escherichia coli 利用大肠杆菌从d -葡萄糖- d -木糖混合物中生产稀有丙烯醛的代谢工程策略

IF 6.1 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry

Pub Date : 2026-10-15 DOI: 10.1021/acs.jafc.6c01629

Zheng-Sheng Shi,Ling Shi,Ling-Jie Zheng,Huidong Zheng,Qiang Guo,Li-Hai Fan

Abstract d-Allulose is a functional sweetener with tremendous potential in food and health industries. Recent studies of its biosynthesis have mainly focused on enzyme catalysis or microbial fermentation using single-sugar substrates. Herein, we constructed a cell factory that coutilizes d-glucose and d-xylose for the efficient biosynthesis of d-allulose. Specifically, the expression of key enzymes in the synthesis pathway, along with the knockout of PtsG, ensured the simultaneous conversion of both sugars to d-allulose. By inactivating PfkA and PfkB and inhibiting Zwf expression, carbon flux from the Embden–Meyerhof–Parnas and pentose phosphate pathways was redirected toward the synthesis pathway. A transhydrogenase cycle was introduced to optimize the cofactor supply, while byproduct pathways were blocked to prevent product loss. Finally, the functionality of the engineered Escherichia coli was confirmed through shake-flask fermentation using wheat straw hydrolysate as the substrate, achieving a titer of 3.33 g/L and a yield of 0.226 g/g.

d-Allulose是一种功能性甜味剂，在食品和保健行业具有巨大的应用潜力。近年来对其生物合成的研究主要集中在单糖底物的酶催化或微生物发酵上。在此，我们构建了一个利用d-葡萄糖和d-木糖进行d-allulose高效生物合成的细胞工厂。具体来说，合成途径中关键酶的表达，以及PtsG的敲除，确保了两种糖同时转化为d-allulose。通过灭活PfkA和PfkB并抑制Zwf的表达，embden - meyerhoff - parnas和戊糖磷酸途径的碳通量被重定向到合成途径。引入转氢酶循环以优化辅因子的供应，同时阻断副产物途径以防止产物损失。最后，以麦草水解液为底物，通过摇瓶发酵证实了工程大肠杆菌的功能，其滴度为3.33 g/L，产率为0.226 g/g。

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引用次数: 0

Novel ModificationsΔI127/L85R in FsSdhD AreInvolved in Differential Resistance to Pydiflumetofen in Fusarium solani 新基因ModificationsΔI127/L85R参与了茄枯菌对嘧螨醚的抗性差异

IF 6.1 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry

Pub Date : 2026-10-15 DOI: 10.1021/acs.jafc.6c08392

Lingling Wei,Liguo Tan,Weimeng Huang,Yinhong Liu,Lu Ren,Xiaojun Zhao

Abstract Lentil root rot (LRR), caused by the soil-borne fungal pathogen Fusarium solani, severely threatens lentil production. Pydiflumetofen (Pyd), a succinate dehydrogenase inhibitor (SDHI) fungicide, is applied to manage diseases caused by Fusarium spp. In this study, the frequency distribution of 50% inhibitory concentration (EC50) of F. solani to Pyd was established, and the mean EC50 value was 0.089 ± 0.0080 μg/mL. A total of 12 Pyd-resistant (PydR) mutants were generated through Pyd-taming, and the resistance frequency was 0.6%. Sequence analysis identified that distinct amino acid substitutions, A85 V in FsSdhC2 or L85R, ΔI127, and D128N in FsSdhD, were associated with moderate to high levels of Pyd resistance. Molecular docking indicated that all mutations significantly reduced the binding affinity between Pyd and the FsSdh complex. These findings advance our mechanistic understanding of SDHI resistance and provide scientific directions for controlling LRR using Pyd.

摘要扁豆根腐病（LRR）是由土传真菌枯萎病（Fusarium solani）引起的，严重威胁着扁豆生产。应用琥珀酸脱氢酶抑制剂（SDHI）杀菌剂吡氟醚（Pydiflumetofen, Pyd）防治镰刀菌（Fusarium spp）引起的疾病，建立了梭兰镰刀菌50%抑制浓度（EC50）的频率分布，平均EC50值为0.089±0.0080 μg/mL。通过pyd驯化共产生12个pyd抗性突变体，抗性频率为0.6%。序列分析发现，不同的氨基酸替换，FsSdhC2或L85R中的A85 V， FsSdhD中的ΔI127和D128N，与中至高水平的Pyd抗性相关。分子对接表明，所有突变都显著降低了Pyd与FsSdh复合物之间的结合亲和力。这些发现促进了我们对SDHI耐药机制的理解，并为利用Pyd控制LRR提供了科学指导。

引用次数: 0

Transcriptomicand Metabolomic Analyses Provide Insightsinto the Effects of Dietary Nanoselenium on Translucent Egg Formationin Laying Hens 转录组学和代谢组学分析揭示了饲粮中纳米硒对蛋鸡半透明蛋形成的影响

IF 6.1 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry

Pub Date : 2026-10-15 DOI: 10.1021/acs.jafc.6c07804

Haojie Gong,Xuemei Ding,Shiping Bai,Keying Zhang,Qiufeng Zeng,Yan Liu,Xiangbing Mao,Jianping Wang

Abstract Eggshell translucency is a common eggshell defect characterized by water-mark-like spots visible to the naked eye under natural light, which can negatively affect consumer acceptance and the market value of eggs. Nanoselenium (Nano-Se) is a novel selenium form with the potential to improve egg quality. In this study, the translucent egg formation and the effects of nano-Se supplementation were investigated using transcriptomic and metabolomic analyses. Translucent eggs showed a higher moisture content and lower eggshell calcium content, along with a thicker and looser mammillary layer and reduced eggshell membrane crude protein and essential amino acid (EAA) contents. Dietary nano-Se reduced translucent egg formation and eggshell moisture, improved egg freshness and mammillary ultrastructure, and altered eggshell membrane amino acid profiles. Transcriptomic analysis revealed the enrichment of calcium signaling- and oxidative phosphorylation-related pathways, while metabolomic analysis highlighted alterations in amino acid biosynthesis and metabolism. Overall, dietary Nano-Se reduced translucent egg formation, accompanied by improved eggshell ultrastructure, reduced eggshell moisture accumulation, and altered eggshell membrane amino acid profiles, which were associated with changes in uterine calcium transport, antioxidant capacity, and amino acid metabolism.

摘要蛋壳半透明是一种常见的蛋壳缺陷，其特征是在自然光下肉眼可见的水渍状斑点，会对鸡蛋的消费者接受度和市场价值产生负面影响。纳米硒（Nano-Se）是一种具有改善鸡蛋品质潜力的新型硒。在本研究中，利用转录组学和代谢组学分析研究了半透明卵的形成和纳米硒补充的影响。半透明鸡蛋水分含量较高，蛋壳钙含量较低，乳层较厚较松，蛋壳膜粗蛋白质和必需氨基酸含量降低。饲粮中添加纳米硒可减少半透明蛋的形成和蛋壳水分，改善鸡蛋的新鲜度和乳头超微结构，并改变蛋壳膜氨基酸谱。转录组学分析揭示了钙信号通路和氧化磷酸化相关通路的富集，而代谢组学分析则强调了氨基酸生物合成和代谢的改变。总体而言，饲粮纳米硒减少了半透明蛋的形成，同时改善了蛋壳超微结构，减少了蛋壳水分积累，改变了蛋壳膜氨基酸谱，这与子宫钙转运、抗氧化能力和氨基酸代谢的变化有关。

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引用次数: 0

A Hydrophobic StructureAssembled Based on Zein Inhibitsthe Occurrence of Wheat Preharvest Sprouting 基于玉米蛋白组装的疏水结构抑制小麦收获前发芽的发生

IF 6.1 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry

Pub Date : 2026-10-15 DOI: 10.1021/acs.jafc.6c11204

Huilan Zhang,Wenke Wang,Caiguo Tang,He Meng,Qianyi Li,Yan Duan,Lifang Wu

Abstract Global climate-related disasters have made preharvest sprouting (PHS), a critical constraint on improving wheat yield and quality. Current prevention strategies primarily rely on breeding new varieties, which face challenges such as prolonged breeding cycles and the difficulty of balancing high yield/quality with sprouting resistance. Meanwhile, existing chemical control agents suffer from inconsistent efficacy, instability, and risks of nonpoint source pollution. In this study, an eco-friendly silicon dioxide nanoparticle/chitosan/zein composite (SCZ) was developed using zein as the natural hydrophobic agent, chitosan (CS) as a base membrane-forming polysaccharide, and silicon dioxide nanoparticles (SiO2 NPs) as filler, each at a final concentration of 1% (w/v). Through optimized ingredient ratios and application methods, field trials demonstrated that SCZ spray achieved over 40% control efficacy against wheat PHS. Scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR) characterization indicated that SCZ formed a compact composite structure with hydrophobic characteristics on the wheat grain surface. This structure blocked water infiltration and thereby inhibited preharvest sprouting. Physiologically, SCZ treatment reduced malondialdehyde (MDA) accumulation and suppressed peroxidase (POD) and alpha-amylase (α-amylase) activation during germination, indicating that SCZ inhibits preharvest sprouting by alleviating oxidative damage and limiting starch mobilization. This research provides theoretical insights into PHS control mechanisms and proposes a novel approach for developing green antisprouting agents, offering significant economic benefits and promising application prospects for sustainable wheat production.

全球气候相关灾害使小麦收获前发芽（PHS）成为制约小麦产量和品质提高的关键因素。目前的防治策略主要依赖于培育新品种，而新品种面临育种周期延长、高产/优质与发芽抗性难以平衡等挑战。同时，现有的化学防治剂存在药效不一致、不稳定和非点源污染风险等问题。本研究以玉米蛋白为天然疏水剂，壳聚糖（CS）为基本成膜多糖，二氧化硅纳米颗粒（SiO2 NPs）为填料，最终浓度为1% (w/v)，制备了一种环保型二氧化硅纳米颗粒/壳聚糖/玉米蛋白复合材料（SCZ）。田间试验表明，通过优化药剂配比和施用方法，SCZ喷雾对小麦小PHS的防治效果达到40%以上。扫描电镜（SEM）和傅里叶变换红外光谱（FTIR）表征表明，SCZ在小麦籽粒表面形成具有疏水特性的致密复合结构。这种结构阻止水分渗入，从而抑制采前发芽。生理上，SCZ处理降低了发芽过程中丙二醛（MDA）的积累，抑制了过氧化物酶（POD）和α-淀粉酶（α-淀粉酶）的激活，表明SCZ通过减轻氧化损伤和限制淀粉动员来抑制采前发芽。本研究为小灵通防治机制的研究提供了理论依据，为开发绿色抑芽剂开辟了一条新途径，为小麦可持续生产提供了显著的经济效益和广阔的应用前景。

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引用次数: 0

Chlorantraniliprole Suppresses the Humoral Immunity in Spodoptera frugiperda Larvae by Downregulating Peptidoglycan Recognition Protein Gene SfPGRP-S5 氯虫腈通过下调肽聚糖识别蛋白基因SfPGRP-S5抑制夜蛾幼虫体液免疫

IF 6.7 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry

Pub Date : 2026-10-14 Epub Date: 2026-10-01 DOI: 10.1021/acs.jafc.6c08585

Qingyan Liu, Wenqi Xie, Jiaxin Liu, Shuhua Liu, Xiaoyue Deng, Qing Li, Yanli Yue, Huabao Chen, Qunfang Yang, Chunxian Jiang

Previous studies have demonstrated that chlorantraniliproleenhancescellular immunity in Spodoptera frugiperda larvae, but its effects on humoral immunity remain unclear. Thus,we investigated the role of this insecticide in modulating humoralresponses, focusing on peptidoglycan recognition proteins (PGRPs)as key mediators. PGRPs are important pattern recognition receptorsthat activate insect innate immunity against microbes. Our findingsrevealed that chlorantraniliprole inhibited humoral immunity in larvae,with the expression of SfPGRP-S5 being significantlyaffected. Functional studies revealed that SfPGRP-S5 knockdown under chlorantraniliprole exposure decreased larval survivalfollowing bacterial infection and downregulated antimicrobial peptide(AMP) genes, while recombinant SfPGRP-S5 activated AMP and prophenoloxidase(PPO) expression. Additionally, the transgenic Drosophila bioassay indicated that SfPGRP-S5 overexpressionenhanced chlorantraniliprole tolerance and reduced bacterial susceptibility.In summary, SfPGRP-S5 plays a critical role in mediatingchlorantraniliprole-induced suppression of humoral immunity in S. frugiperda larvae. This study reveals the differentialimmune regulation by insecticides and provides new insights for understandingtheir sublethal effects.

摘要以往的研究表明，氯虫腈能增强夜蛾幼虫的细胞免疫，但其对体液免疫的影响尚不清楚。因此，我们研究了这种杀虫剂在调节体液反应中的作用，重点研究了肽聚糖识别蛋白（PGRPs）作为关键介质的作用。PGRPs是一种重要的模式识别受体，可激活昆虫对微生物的先天免疫。我们的研究结果表明，氯虫腈抑制了幼虫的体液免疫，并显著影响了SfPGRP-S5的表达。功能研究显示，暴露于氯虫腈下，SfPGRP-S5基因敲除降低了细菌感染后的幼虫存活率，下调了抗菌肽（AMP）基因，而重组SfPGRP-S5基因激活了AMP和酚氧化酶（PPO）的表达。此外，转基因果蝇的生物测定表明，SfPGRP-S5过表达增强了氯虫腈的耐受性，降低了细菌的敏感性。综上所述，SfPGRP-S5在氯虫腈诱导的frugiperda幼虫体液免疫抑制中起关键作用。本研究揭示了杀虫剂对免疫调节的差异，为了解杀虫剂的亚致死效应提供了新的见解。

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引用次数: 0

Comparing the Impacts of a Biopesticide and a Synthetic Pesticide on Potato Production: A Multi-Omics Analysis of Carvacrol and Dimethomorph 生物农药与合成农药对马铃薯生产的影响比较：马铃薯地霉霉多组学分析

IF 6.7 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry

Pub Date : 2026-10-14 Epub Date: 2026-09-30 DOI: 10.1021/acs.jafc.6c09742

Weitao Wang, Wenyuan Huang, Sarah J. Knight, Karin Müller, Long Cheng, Fan Xiang, Jiahuan Long, Melanie Kah

Long-term application of synthetic pesticides for potatolate blightcontrol has raised many ecological concerns, while broader impactsof biopesticides on production systems remain unclear. Field trialscompared the effects of pesticides dimethomorph (synthetic) and carvacrol(biological) against an untreated control in a potato production system,integrating untargeted metabolomics, metabarcoding and quality traitanalyses. Compared with the control group, both pesticide treatmentsupregulated tuber lipid metabolism but downregulated leaf defense-associatedmetabolism. For rhizosphere communities, while both treatments reducedbeneficial bacterial taxa, carvacrol exerted less suppressive effectsthan dimethomorph and resulted in superior pathogen suppression. Thesechanges were associated with enhanced potato quality under carvacroltreatment, with starch, flavonoid, and vitamin C contents each morethan 10% higher than under dimethomorph. This study highlights theimportance of evaluating both productivity end points and soil ecologicalconsequences in field conditions, providing novel insights into theintegration of biopesticides in sustainable crop production.

长期使用合成农药防治马铃薯晚疫病引起了许多生态问题，而生物农药对生产系统的广泛影响尚不清楚。田间试验综合了非靶向代谢组学、元条形码和品质性状分析，比较了在马铃薯生产系统中，杀虫剂菊硫醚（合成）和香芹酚（生物）与未经处理的对照的效果。与对照组相比，两种农药处理均上调了块茎脂质代谢，下调了叶片防御相关代谢。对于根际群落，虽然两种处理都减少了有益细菌的分类群，但香芹酚的抑制作用比吡虫啉小，对病原体的抑制效果更好。这些变化与香芹酚处理下马铃薯品质的提高有关，淀粉、类黄酮和维生素C含量均比吡虫啉处理下高10%以上。这项研究强调了在田间条件下评估生产力终点和土壤生态后果的重要性，为生物农药在可持续作物生产中的整合提供了新的见解。

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引用次数: 0

Hyperoside Derived from Actinidia arguta Bee Pollen Alleviates Zearalenone-Induced Hepatotoxicity and Oxidative Stress in Piglets via the PI3K/AKT Signaling Pathway 猕猴桃花粉提取的金丝桃苷通过PI3K/AKT信号通路减轻玉米赤霉烯酮诱导的仔猪肝毒性和氧化应激

IF 6.7 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry

Pub Date : 2026-10-14 Epub Date: 2026-10-02 DOI: 10.1021/acs.jafc.6c05739

Nannan Liu, Dongfei You, Yunfei Jiang, Tianyu Han, Guangliang Shi

Zearalenone (ZEA) is a prevalent mycotoxin inducing hepatotoxiceffects. Hyperoside (HYP), a flavonoid derived from Actinidia arguta bee pollen, exhibits hepatoprotectiveproperties against ZEA; however, the molecular mechanisms underlyingthis protection remain incompletely characterized. This study integratedin vivo piglet models, in vitro porcine hepatocytes, network pharmacology,and molecular docking to characterize the molecular targets of HYP.The results demonstrated that HYP significantly attenuated ZEA-inducedhepatic lesions and oxidative stress. Computational analyses pinpointedthe PI3K/AKT signaling pathway as the core target of HYP. In vitrovalidation revealed that HYP phenocopied the AKT activator SC79 insuppressing ZEA-induced hepatocyte apoptosis, an efficacy partiallyabrogated by the PI3K inhibitor LY294002. Conclusively, HYP amelioratesZEA-induced hepatotoxicity by activating the PI3K/AKT axis to mitigateoxidative stress and apoptosis, underscoring its translational potentialas a functional detoxifying additive for food and feed applications.

玉米赤霉烯酮（ZEA）是一种常见的引起肝毒性作用的真菌毒素。金丝桃苷（HYP）是一种从猕猴桃蜂花粉中提取的类黄酮，具有抗ZEA的肝保护作用；然而，这种保护的分子机制仍然不完全清楚。本研究结合仔猪体内模型、离体猪肝细胞、网络药理学、分子对接等方法对HYP的分子靶点进行了表征，结果表明HYP能显著减轻zea诱导的肝脏病变和氧化应激。计算分析确定PI3K/AKT信号通路是HYP的核心靶点，体外验证表明，HYP在抑制zea诱导的肝细胞凋亡中表现出AKT激活剂SC79的表型，这一作用部分被PI3K抑制剂LY294002所取代。总之，HYP通过激活PI3K/AKT轴来减轻氧化应激和细胞凋亡，从而改善了zea诱导的肝毒性，强调了其作为食品和饲料中功能性解毒添加剂的转化潜力。

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引用次数: 0