Archives of Physiology and Biochemistry最新文献

Background and purpose: This study, based on the network pharmacology approach, aims to systematically explore the potential molecular mechanism of Cangshu Diantong Pill in treating type 2 diabetes (T2DM), and particularly emphasises the theoretical correlation between its mechanism of action and the modern metabolic abnormality diagnostic indicators.

Methods: The study first screened the active components in Cangshu Diantong Pill that have pharmacokinetic characteristics through databases such as TCMIP, it integrated disease databases to obtain T2DM-related targets and constructed a drug-disease intersection target network. Core targets included AKT2, TNF, ADIPOQ, IGF1, etc., all of which are key biomarkers in current metabolic abnormality diagnostics.

Results: Pathway enrichment showed that Cangshu Diantong Pill mainly exerts its effects by regulating signalling pathways.

Conclusion: The study suggests that Cangshu Diantong Pill's intervention on T2DM may manifest as a synergistic regulation of multiple metabolic abnormality links, and its theoretical action pathway is highly consistent with the modern metabolic diagnostic system.

Background: The occurrence and development of intestinal diseases are closely related to intestinal metabolic disorders.

Purpose: This study focuses on the metabolic analysis of intestinal diseases under nutritional intervention as the core perspective, aiming to construct a prediction model for the tolerance of patients undergoing non-sedating colonoscopy.

Results: The results showed that the completion rate of the examination for the study subjects was 89.93%. Those who did not complete the examination mainly suffered from pain intolerance and poor intestinal preparation. Univariate analysis indicated that age, grade III anxiety, history of constipation, and the operator had statistical effects on the completion rate. Age and history of constipation affected the examination completion time. Multivariate analysis confirmed that grade III anxiety, history of constipation.

Conclusion: The study provides clinical evidence and intervention targets for using nutritional intervention to regulate intestinal metabolic status and enhance the tolerance of non-sedating colonoscopy.

ABSTARCTThe present study investigates the neuroprotective effects of Cannabidiol (CBD) in olanzapine/high-fat diet (HFD) treated adult male Wistar rats. Animals weighing 160-180 g were divided into five groups (n = 7). They include Untreated, HFD+Olan, Met + HFD+Olan, CBD (10 mg/kg)+HFD+Olan, and CBD (25 mg/kg)+HFD + Olan. Olanzapine and metformin were administered as 5 mg/kg and 20 mg/kg, respectively. Cognitive functions, brain biochemical markers, histology and immunohistochemistry were determined. 2 weeks cotreatment of olanzapine/HFD induced cognitive impairment evidenced by significant reduction in Object location memory (OLM) from 3.5 ± 0.75 to -39 ± 8.57% and Object recognition memory (ORM) indices from 21.47 ± 12.68 to -30.30 ± 4.29%, increased serum amyloid beta 1-42 and GFAP hypothalamic and prefrontal cortex expressions. CBD comparable to metformin ameliorated the induced cognitive impairment. CBD possesses remarkable cognitive preserving activity which is associated with decreased lipid peroxidation and down regulation of GFAP/ACHE pathway.

Background: Cellular ageing is closely related to various age-related diseases, and Klotho protein, as an anti-ageing related factor, plays an important role in delaying ageing by regulating cellular function and metabolism.

Purpose: This study aims to explore the role of Klotho protein in exercise intervention and its relationship with age-related metabolism.

Methods: Study the effects of exercise intervention on serum Klotho protein levels, Klotho gene methylation status, ageing related β - galactosidase (SA - β - Gal) activity, and ageing metabolic indicators, revealing the molecular mechanism of exercise delaying cellular ageing. The experiment was divided into a control group and an exercise intervention group, with an intervention period of 12 weeks.

Results: Exercise intervention significantly improved oxidative stress indicators and blood lipid metabolism.

Conclusion: Exercise intervention delays cellular ageing by upregulating serum Klotho protein levels and reducing gene methylation levels, inhibiting SA - β - Gal activity, improving oxidative stress and lipid metabolism.

Background: Exercise fatigue is an important factor affecting exercise performance and recovery. Studies have found that AMPK (adenosine monophosphate activated protein kinase) plays a key role in energy metabolism and exercise adaptation.

Purpose: The study aims to explore role of AMPK protein macromolecules in alleviating exercise fatigue and the characteristics of their signalling pathways.

Methods: This article uses a rat model for experiments, extracts and detects RNA from muscle tissue, and uses real-time fluorescence quantitative PCR to analyse the expression level of AMPK and evaluate its activation status. By analysing the expression effect of AMPK related protein signalling pathway, explore its impact on exercise fatigue.

Results: The exercise intervention group of rats regulated by AMPK showed significant improvement in exercise fatigue, significant increase in energy metabolism indicators, and enhanced immune function.

Conclusion: The results indicate that AMPK protein macromolecules effectively alleviate exercise fatigue and improve energy metabolism and immune function by activating related signalling pathways.

Background: With the intensification of population ageing, the incidence of bone metabolism-related diseases such as osteoporosis has significantly increased, becoming an important factor affecting the quality of life of the elderly.

Materials and methods: A total of 120 patients with rotator cuff tear (RCT) were selected as the research subjects. There were 65 male patients and 55 female patients. The aim of this study was to explore the predictive value of bone metabolism disease examinations and changes in proximal humeral bone density for intraoperative anchor loosening. The predictive value of proximal humeral bone density for intraoperative anchor loosening was analysed using the ROC curve.

Results: The increase in age and the decrease in bone density are closely related to risk of intraoperative anchor loosening.

Conclusion: The combination of bone metabolism disease examinations and proximal humeral bone density detection can provide important basis for clinical preoperative prediction of anchor loosening risk and formulation of individualised treatment plans.

Objective: This study aimed to explore the role of LINC00472 in type 2 diabetes mellitus (T2DM).

Methods: Serum LINC00472 expression was detected in 34 patients with T2DM and 45 healthy controls via RT-qPCR, with ROC curve analysing its diagnostic value. Mouse pancreatic β-cell line INS-1 was induced with high glucose (HG) to establish HG-INS-1 model. si-LINC00472 and miR-320b inhibitor were transfected into HG-INS-1. CCK-8, flow cytometry, and ELISA kits were used to detect cell viability, apoptosis, insulin level, and oxidative stress indicators. Subcellular fractionation, dual-luciferase reporter, and RIP assays verified the interaction between LINC00472 and miR-320b.

Results: Serum LINC00472 was elevated in T2DM patients, with AUC = 0.882, 73.81% sensitivity, and 94.29% specificity. Silencing LINC00472 enhanced HG-INS-1 viability, reduced apoptosis, increased insulin, decreased ROS/MDA, and increased GSH. LINC00472 was mainly cytoplasmic and targeted miR-320b.

Conclusion: LINC00472 is a potential T2DM biomarker.

Background: Colorectal cancer (CRC) is a highly prevalent and fatal malignancy worldwide. Despite advancements in early screening techniques and treatments, the prognosis for patients remains suboptimal. Studies have shown that metal ions play crucial roles in the occurrence, progression, and treatment of CRC.

Method: Regulating the concentrations of specific metal ions within tumour cells can promote cancer cell death and increase the effectiveness of chemotherapy, radiotherapy, targeted therapy, and immunotherapy.

Results: This article reviews the relationships between metal ions such as iron, copper, potassium, calcium, magnesium, and zinc and CRC, summarising recent research progress from mechanistic studies to clinical applications. Magnesium ions inhibit CRC development and metastasis by regulating various signalling pathways can either promote or inhibit tumour-associated gene expression.

Conclusion: Therefore, modulating the concentrations of relevant metal ions within tumour cells could be a potential therapeutic direction for CRC, providing new theoretical foundations and strategies for clinical treatment.

Chronic sinusitis with nasal polyps (CRSwNP) has a relatively high recurrence rate, seriously affecting the quality of life of patients. Neuroinflammation interacts with the persistent inflammatory state of the nasal mucosa. This study aims to explore the application value of medical thermal imaging technology in predicting postoperative recurrence of nECRSwNP, and to evaluate potential biomarkers and efficacy assessment methods for neuroinflammation treatment, with the expectation of providing new strategies for reducing postoperative recurrence rates and improving the quality of life. Medical thermal imaging technology can monitor the temperature changes of nasal mucosa in real time and promptly detect alterations in inflammatory activity. The levels of EOS and cytokines in peripheral blood can serve as potential biomarkers for evaluating the status of neuroinflammation, which is helpful for identifying patients with a higher risk of recurrence, thereby enabling timely adjustment of treatment plans and improving the pertinence and effectiveness of neuroinflammation treatment.

Lipoprotein metabolic regulation plays a vital role in human health and disease, with its abnormalities closely associated with the onset and progression of various disorders. In cases of lymphadenopathy, this study aimed to develop a linear model based on multimodal ultrasound parameters and lipoprotein metabolic regulation, predicting changes in intraluminal and perilymphatic tissue stiffness during cervical lymphadenopathy to provide clinical guidance for assessing lymph node characteristics. All patients underwent multimodal ultrasound examinations including conventional 2D ultrasound, colour Doppler ultrasound, and elastography. The linear model developed based on these factors demonstrated high predictive power, indicating that changes in lipoprotein metabolism are closely linked to the pathophysiological processes of lymph nodes. In lymphadenopathy, lipoprotein metabolic regulation affects local inflammatory responses and extracellular matrix remodelling, thereby influencing lymph node stiffness and function. Additionally, lipoprotein regulation indirectly impacts lymph node hardness by modulating angiogenesis and extracellular matrix remodelling.