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The Clinical Value of Detecting the Level of Exfoliated Cells in Pleural Effusion by Flow Cytometry in the Differential Diagnosis of Non-Small Cell Lung Cancer and Benign Lung Diseases 流式细胞术检测胸腔积液脱落细胞在非小细胞肺癌癌症与良性肺部疾病鉴别诊断中的临床价值
Pub Date : 2023-05-31 DOI: 10.26689/jcnr.v7i3.4849
Yang Yu, Yiyang Li, Chengbi Tong, Xiaomeng Zheng, Chaoqi Yang
Objective: To explore the value of flow cytometry (FCM) in detecting the level of exfoliated cells in pleural effusion in the differential diagnosis of non-small cell lung cancer and benign lung diseases. Methods: Clinical data of patients with non-small cell lung cancer who were hospitalized in Hebei hospital from June 2019 to March 2022 were collected. A total of 98 patients were included, and 63 patients with alveolar lung disease were screened during the same period, and the two groups of patients were analyzed. Results: Compared with alveolar lung disease group, FCM detection and analysis showed that the level of exfoliated cells in the pleural effusion of non-small cell lung cancer (NSCLC) patients was 99 (3-969)/100,000, and patients with alveolar lung disease was 4 (0 ~ 19)/100,000. Additionally, compared with the alveolar lung disease group, the level of exfoliated cells in the pleural effusion of patients with non-small cell lung cancer (NSCLC) was significantly increased (P<0.001). The diagnostic efficacy of FCM for detecting pleural fluid exfoliated cells in non-small cell lung cancer was assessed using ROC curves and using 95% CI (-11.1, -13.2) with a sensitivity of 0.75 and specificity of 0.94, and the diagnostic efficacy of FCM for detecting pleural fluid exfoliated cells in alveolar lung disease was assessed using 95% CI (-11.1, -13.2) with a sensitivity of 0.71 and specificity of 0.87. Conclusion: Flow cytometry has a wider range of clinical applications, simple operation, low cost, and high sensitivity, which makes it of great significance in disease diagnosis.
目的:探讨流式细胞术(FCM)检测胸腔积液脱落细胞水平对非小细胞肺癌癌症和良性肺部疾病的鉴别诊断价值。方法:收集2019年6月至2022年3月在河北省医院住院的非小细胞肺癌癌症患者的临床资料。共纳入98例患者,同期筛查63例肺泡肺病患者,并对两组患者进行分析。结果:流式细胞术检测分析非小细胞肺癌(NSCLC)患者胸腔积液脱落细胞水平为99(3~699)/10万,与肺泡疾病组相比,肺泡疾病组为4(0~19)/10万。与肺泡疾病组相比,非小细胞肺癌(NSCLC)胸腔积液中脱落细胞含量显著升高(P<0.001)。采用ROC曲线和95%CI(-11.1,-13.2),FCM检测肺泡疾病中胸膜液脱落细胞的诊断效果采用95%CI(-11.1,-13.2)进行评估,敏感性为0.71,特异性为0.87。结论:流式细胞术具有临床应用范围广、操作简便、成本低、灵敏度高等特点,在疾病诊断中具有重要意义。
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引用次数: 0
The Role and Mechanism of LncRNA-p21 in Regulating Gastric Cancer Metastasis by Mediating Wnt/β-Catenin Signaling Pathway LncRNA-p21介导Wnt/β-Catenin信号通路调控胃癌转移的作用及机制
Pub Date : 2023-05-31 DOI: 10.26689/jcnr.v7i3.4862
Peng Liu, Haizhe Chang, Xinyu Peng, Xiuqin Zhang, Jianjun Zhang, Jiusong Luan
Objective: To study the mechanism of lncRNA p21 inhibiting the growth and metastasis of human gastric cancer SGC7901/GES-1 cells by mediating the Wnt/β-catenin signaling pathway. Methods: Lentiviral overexpression of lncRNA-p21 in human gastric cancer SGC7901/GES-1 cell transfections was observed and analyzed for in vitro migration, invasion, cell morphology and proliferation. Besides. Wnt/β-catenin signaling pathway was tested for direct involvement in lncRNA-p21-mediated inhibition of gastric cancer cell growth and proliferation. Wnt/β-catenin signaling pathway was validated using Li-C1. Results: Gastric cancer SGC7901/GES-1 cells in the overexpression of lncRNA-p21 showed changes in stellate morphology, low invasion, or spindle-shaped morphology. LncRNA-p21 inhibited the growth and proliferation of gastric cancer SGC7901/GES-1 cells both in vivo and in vitro, and Wnt/β-catenin signaling pathway mediated the proliferation, invasion, and migration of lncRNA-p21 on gastric cancer SGC7901/GES-1 cells. Conclusion: LncRNA-p21 can inhibit the growth and metastasis of gastric cancer SGC7901/GES-1 cells in vitro and in vivo, and the inhibition of lncRNA p21 is mainly mediated by inhibiting the Wnt/β-catenin signaling pathway.
目的:探讨lncRNAp21介导Wnt/β-catenin信号通路抑制人癌症SGC7901/GES-1细胞生长转移的机制。方法:观察lncRNA-p21慢病毒在人癌症SGC7901/GES-1细胞转染中的过度表达,并分析其体外迁移、侵袭、细胞形态和增殖。此外检测Wnt/β-catenin信号通路直接参与lncRNA-p21-介导的对癌症细胞生长和增殖的抑制。利用Li-C1对Wnt/β-catenin信号通路进行了验证。结果:癌症SGC7901/GES-1细胞在lncRNA-p21的过度表达中表现出星状形态、低侵袭或棘状形态的改变。LncRNA-p21在体内外均能抑制癌症SGC7901/GES-1细胞的生长和增殖,Wnt/β-catenin信号通路介导LncRNA-p21对癌症SGC7901-GES-1细胞增殖、侵袭和迁移。结论:LncRNA-p21在体内外均可抑制癌症SGC7901/GES-1细胞的生长和转移,其抑制LncRNA-p21的作用主要通过抑制Wnt/β-catenin信号通路来实现。
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引用次数: 0
Risk Factors of Neonatal Medical Adhesive-Related Skin Injury and Management of High-Risk Nodes 新生儿医用粘合剂性皮肤损伤的危险因素及高危淋巴结的处理
Pub Date : 2023-05-31 DOI: 10.26689/jcnr.v7i3.4959
Sisi Hu, X. Huang, Dan Wang
Objective: To analyze the risk factors of neonatal medical adhesive-related skin injury and put forward targeted preventive measures, so as to provide reference for the care and prevention of neonatal medical adhesive-related skin injuries. Methods: Using the convenience sampling method, 262 neonates admitted to the neonatal intensive care unit (NICU) of a tertiary general hospital in Wenzhou from April 2021 to May 2022 were selected as the study subjects. The incidence of medical adhesive-related skin injuries in these neonates was retrospectively analyzed. Results: Among the 262 children, 43 cases had skin injuries, with an incidence rate of 16.4%. Single factor analysis showed that the occurrence of medical adhesive-related skin injury was related to gestational age, weight, electrocardiogram (ECG) monitoring, venous access, ambient temperature, and mechanical ventilation (P < 0.05). Multivariate logistic regression showed that gestational age, ECG monitoring, and ambient temperature were independent risk factors of medical adhesive-related skin injury (OR values were 0.700, 0.431, and 6.365, respectively). Conclusion: The high incidence of neonatal medical adhesive-related skin injury may be caused by one or more factors. Clinical measures should be taken, such as selecting the appropriate type of adhesive according to gestational age and using skin-protecting membrane, minimizing ECG monitoring, etc., to prevent the occurrence of neonatal medical adhesive-related skin injury.
目的:分析新生儿医用胶粘剂相关皮肤损伤的危险因素,提出有针对性的预防措施,为新生儿医用胶粘剂皮肤损伤的护理和预防提供参考。方法:采用方便抽样法,选取2021年4月至2022年5月入住温州市某三级综合医院新生儿重症监护室(NICU)的262名新生儿作为研究对象。回顾性分析了这些新生儿中与医用粘合剂相关的皮肤损伤的发生率。结果:262例儿童中,43例发生皮肤损伤,发生率为16.4%。单因素分析显示,医用粘合剂相关皮肤损伤的发生与胎龄、体重、心电图监测、静脉通路、环境温度和机械通气有关(P<0.05)。多因素logistic回归显示,心电图监测和环境温度是医用粘合剂相关皮肤损伤的独立危险因素(OR值分别为0.700、0.431和6.365)。结论:新生儿医用粘合剂相关皮肤损伤的高发可能是由一个或多个因素引起的。应采取临床措施,如根据孕龄选择合适的粘合剂类型并使用皮肤保护膜、尽量减少心电图监测等,预防新生儿医用粘合剂相关皮肤损伤的发生。
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引用次数: 0
Analysis of the Implementation of Integrated Healthcare Management in Preoperative Preparation 综合医疗管理在术前准备中的实施分析
Pub Date : 2023-05-31 DOI: 10.26689/jcnr.v7i3.4831
Huiling Liu, Xuling Li, Xinyi Liu, Yuhua Ma, Haomin Li, Cuiqing Liu
Objective: To strengthen preoperative preparation management and reduce preoperative preparation defects. Methods: We set up a special medical care management team to investigate the preoperative preparation in surgical departments, analyze the causes of the shortcomings in preoperative preparation, and propose countermeasures. The medical staff jointly formulated plans related to preoperative preparation management including clear outline of responsibilities, strengthening of training, integrated medical care, timely feedback, and continuous improvement. Besides, the preoperative preparation of patients before and after the improvement measures were compared using statistical analysis. Results: The rate of inadequate preoperative preparation was 11.14 per thousand before the integrated management model was implemented. After the implementation of the integrated management model, the rate of inadequate preoperative preparation decreased to 2.99 per thousand, and the difference was statistically (P < 0.05). Conclusion: The integrated management of preoperative preparation can help reduce the rate of preoperative complications and ensure the safety of surgery.
目的:加强术前准备管理,减少术前准备缺陷。方法:成立专门的医疗护理管理小组,对外科的术前准备情况进行调查,分析术前准备不足的原因,并提出对策。医务人员共同制定了与术前准备管理相关的计划,包括明确职责大纲、加强培训、一体化医疗、及时反馈和持续改进。此外,对患者术前准备情况及改善措施前后进行统计分析比较。结果:在实施综合管理模式之前,术前准备不足的比率为11.14/1000。实施一体化管理模式后,术前准备不足率降至千分之2.99,差异有统计学意义(P<0.05)。结论:术前准备一体化管理有助于降低术前并发症发生率,确保手术安全。
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引用次数: 0
Nano-Sustained CO-Releasing Molecules Alleviates Cyclosporin-A-Induced Nephrotoxicity and Renal Fibrosis by Inhibiting NLRP3 Inflammasome-Mediated TGF-β/Smad Signaling Pathway 纳米持续co释放分子通过抑制NLRP3炎症小体介导的TGF-β/Smad信号通路减轻环孢素a诱导的肾毒性和肾纤维化
Pub Date : 2023-05-31 DOI: 10.26689/jcnr.v7i3.4806
J. Ji, Zhao Bi, Ling Tian, Qian Zhang, Shuhua Hou, Song Li
Objective: To investigate the effect nano-sustained CO-releasing molecules on cyclosporin-A (CsA)-induced nephrotoxicity by inhibiting the NLRP3 inflammasome-mediated TGF-β/Smad signaling pathway. Methods: 3×105 cell/mL human renal tubular epithelial cells (HK-2) and mouse primary cultured renal tubular epithelial cells (RTECs) were cultured under an inverted microscope and incubated with 10% DMEM and 0.25% β2M in NaCl solution for 3 h. HK-2 and RTECs were divided into 5 complex numbers. MTT assay was used to detect the relative proliferation level of one of the HK-2 cells and calculate the multiplication ratio. Results: The nano-sustained CO-releasing molecules CS-CO had a strong protective effect on the kidney. HK-2 and RTECs cells were treated with siRNA, inhibitors, and NLRP3 knockout mice, and the changes in cell activity and expression of intracellular inflammatory factors were studied. The expression of TGF-β1/Smad signaling pathway related proteins in HK-2 and RTECs was detected by ELISA, western blot, immunofluorescence, and other techniques. Conclusion: SMA/CORM2 alleviates CsA-induced renal fibrosis by inhibiting NLRP3 inflammasome-mediated TGF-β/Smad signaling pathway.
目的:探讨纳米CO缓释分子通过抑制NLRP3炎症小体介导的TGF-β/Smad信号通路对环孢菌素A(CsA)诱导的肾毒性的影响。方法:在倒置显微镜下培养3×105细胞/mL人肾小管上皮细胞(HK-2)和小鼠原代培养的肾小管上皮上皮细胞(RTEC),并与10%DMEM和0.25%β2M在NaCl溶液中孵育3h。MTT法检测其中一个HK-2细胞的相对增殖水平并计算增殖率。结果:纳米CO缓释分子CS-CO对肾脏有较强的保护作用。用siRNA、抑制剂和NLRP3敲除小鼠处理HK-2和RTEC细胞,并研究细胞活性和细胞内炎症因子表达的变化。采用ELISA、蛋白质印迹、免疫荧光等技术检测HK-2和RTEC中TGF-β1/Smad信号通路相关蛋白的表达。结论:SMA/CORM2通过抑制NLRP3炎症小体介导的TGF-β/Smad信号通路,减轻CsA诱导的肾纤维化。
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引用次数: 0
Clinical Observation of Non-Staining Circular Capsulorhexis for Hypermature Cataract 未染色环形撕囊术治疗超成熟白内障的临床观察
Pub Date : 2023-05-31 DOI: 10.26689/jcnr.v7i3.4985
Lizhao Wang, Rong Li
Objective: To explore the circular capsulorhexis method and its clinical effect in phacoemulsification of hypermature cataract without staining. Methods: In this prospective randomized case-control study, 22 patients (22 eyes) with hypermature cataract were randomly divided into a staining agent group (10 cases, 10 eyes) and a non-staining agent group (12 cases, 12 eyes). Both groups underwent phacoemulsification with foldable intraocular lens implantation under topical anesthesia. In the staining agent group, 0.5% indocyanine green was used to stain the anterior lens capsule to assist in circular capsulorhexis; in the non-staining agent group, after puncturing the anterior lens capsule and aspirating the liquefied cortical material, the capsular bag was filled with viscoelastic agent to increase the contrast prior to circular capsulorhexis. The operability of the anterior capsule without staining in capsulorhexis was observed, and the effect of capsulorhexis, diameter of capsulorhexis opening, and operative time were compared and analyzed between the two groups. Results: Both groups successfully underwent circular capsulorhexis, with a success rate of 100%. The diameters of capsulorhexis opening in the staining agent group and non-staining agent group were 5.68 ± 0.15 mm and 5.54 ± 0.16 mm, respectively, without any statistical difference between the groups (P > 0.05); the operative time of the staining agent group (40.40 ± 5.42 s) was shorter than that of the non-staining agent group (50.92 ± 3.97 s), and the difference was statistically significant (P < 0.001). In both groups, phacoemulsification was successful, and intraocular lens were implanted into the capsular bags; their visual acuity showed significant improvement during the 1-month follow-up period after surgery, without any surgical complications. Conclusion: In hypermature cataract, injecting viscoelastic agent into the capsular bag for volume replacement, given a lack of capsule staining agent, can increase the contrast for capsulorhexis and reduce the risk of large capsular bag collapse on the zonular ligament. Thus, it is considered safe and effective. Circular capsulorhexis is conducive to a smooth phacoemulsification procedure.
目的:探讨环形撕囊法在无染色超成熟白内障超声乳化术中的应用效果。方法:采用前瞻性随机病例对照研究,将22例(22眼)高度成熟性白内障患者随机分为染色剂组(10例,10眼)和非染色剂组(12例,12眼)。两组均在表面麻醉下行超声乳化术合并折叠式人工晶状体植入术。染色剂组采用0.5%吲哚菁绿染色前晶状体囊,辅助圆形撕囊术;非染色剂组穿刺晶状体前囊,抽吸液化的皮质物质后,在囊袋内填充粘弹性剂,增加造影剂,然后进行圆形撕囊。观察前囊无染色撕囊术的可操作性,并比较分析两组撕囊效果、撕囊口直径、手术时间。结果:两组均成功行环形撕囊术,成功率均为100%。染色剂组和未染色剂组撕囊口直径分别为5.68±0.15 mm和5.54±0.16 mm,组间差异无统计学意义(P < 0.05);染色剂组手术时间(40.40±5.42 s)短于未染色剂组(50.92±3.97 s),差异有统计学意义(P < 0.001)。两组超声乳化手术均成功,人工晶状体植入囊袋内;术后随访1个月,视力明显改善,无手术并发症。结论:对于高度成熟的白内障,在缺乏囊染色剂的情况下,在囊袋内注射粘弹性剂进行体积置换,可以提高撕囊造影剂的造影率,降低韧带上大囊袋塌陷的风险。因此,它被认为是安全有效的。圆形撕囊有利于超声乳化手术的顺利进行。
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引用次数: 0
Application of Heuristic Method to the Clinical Teaching of Internal Medicine 启发式教学法在内科临床教学中的应用
Pub Date : 2023-05-26 DOI: 10.26689/jcnr.v7i3.4974
Y. Ni, Hongyuan Bai, Haitao Shi
There is an old saying, “Give a man a fish, and he will eat for a day. Teach a man to fish, and he will eat for the rest of his life.” In clinical teaching, students should not only be taught about diseases, but their intelligence should also be cultivated, along with their analytical, comprehension, and independent learning skills. The ability to solve problems enables students to think independently and acquire knowledge. This is known as the heuristic method of teaching. In this study, we mainly analyze the application value of the heuristic method in the clinical teaching of internal medicine.
俗话说:“给人一条鱼,他可以吃一天。”教一个人钓鱼,他就能吃一辈子。”在临床教学中,不仅要培养学生对疾病的认识,更要培养学生的智力,培养学生的分析能力、理解能力和独立学习能力。解决问题的能力使学生能够独立思考和获取知识。这就是所谓的启发式教学法。本文主要分析了启发式教学法在内科临床教学中的应用价值。
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引用次数: 0
Experience in Surgical Coordination of Type III Cesarean Scar Pregnancy Removal with Combined Assistance of Hysteroscopy and Laparoscopy 宫腔镜与腹腔镜联合配合Ⅲ型剖宫产瘢痕妊娠摘除术的经验
Pub Date : 2023-05-22 DOI: 10.26689/jcnr.v7i3.4907
Fang Zhou, Jinfeng Tan, Y. Li, Qinfang Huang, Y. Teng
: Objective: This paper aims to summarize the operative nursing coordination essentials of type III cesareans car pregnancy removal. Methods: Six patients were recruited for this study, and the patient’s condition was fully evaluated before the operation. In addition, the personnel, environment, and materials were well prepared before the operation, and the preparation of special intraoperative instruments and equipment was perfected. Results: The operation is successfully performed in all six patients. Each surgery lasted 70 to 120 minutes with an average duration of 90 minutes. Postoperative blood loss was about 100-500 ml. Postoperative recovery was good without complications in all the patients. Conclusion: In summary, laparoscopic removal of gestational tissue from type III cesareans car pregnancy in conjunction with hysteroscopy ensures compete removal of gestational tissue, while avoiding damaging the surrounding organs and tissues, thus greatly avoiding common complications which usually occur during the surgery. Effective surgical coordination is helpful to further improve the success rate of the operation.
目的:总结Ⅲ型剖宫产车妊娠摘除术的手术护理配合要点。方法:本研究招募了6名患者,并在手术前对患者的病情进行了全面评估。此外,术前做好了人员、环境和材料准备,完善了术中专用仪器设备的准备。结果:6例患者均成功完成了手术。每次手术持续70至120分钟,平均持续时间为90分钟。术后出血量约为100-500ml。所有患者术后恢复良好,无并发症。结论:总之,腹腔镜下切除III型剖宫产车妊娠期妊娠组织,结合宫腔镜检查,既能确保妊娠组织的竞争性切除,又能避免对周围器官和组织的损伤,从而大大避免了手术中常见的并发症。有效的手术配合有助于进一步提高手术的成功率。
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引用次数: 0
Patient Safety Risks and Measures for Pediatric Neurosurgery Nursing 小儿神经外科患者安全风险及护理对策
Pub Date : 2023-05-22 DOI: 10.26689/jcnr.v7i3.4961
Lili Jiang, Weifang Yang, Song Zhang
The aim of this study was to determine the patient safety risks and measures for pediatric neurosurgery nursing. A total of 564 pediatric patients admitted to the hospital from June 2020 to June 2023 under the neurosurgery department were included in this study. We analyzed the safety incidents in pediatric neurosurgery nursing and their causes and proposed corresponding measures for pediatric neurosurgery nursing in hope to reduce the occurrence of patient safety incidents in pediatric neurosurgery nursing and establish harmonious nurse-patient relationships.
本研究的目的是确定儿童神经外科护理的患者安全风险和措施。本研究共纳入2020年6月至2023年6月在该院神经外科住院的564例儿科患者。通过对小儿神经外科护理安全事件及其原因的分析,提出小儿神经外科护理的相应措施,以期减少小儿神经外科护理中患者安全事件的发生,建立和谐的护患关系。
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引用次数: 0
Research Progress of Traditional Chinese and Western Medicine Dressing Change on Wound Healing After Anal Fistula Surgery 中西医换药对肛瘘术后创面愈合的影响研究进展
Pub Date : 2023-04-20 DOI: 10.26689/jcnr.v7i3.4877
Xiaona Miao, Jia-min Hang
With the change of people’s lifestyle and diet, the incidence of anorectal diseases is increasing year by year. Anal fistula is a common anorectal disease. Because it cannot heal by itself, surgery has become the main treatment method. Due to the particularity of wound location and physiological structure, the wound is easily contaminated by bacteria, so dressing change after surgery plays a decisive role in wound healing. Modern western medicine and traditional Chinese medicine have different dressing changing methods respectively. In this paper, we reviewed commonly used dressing changing methods of traditional Chinese and western medicine after anal fistula surgery.
随着人们生活方式和饮食习惯的改变,肛肠疾病的发病率逐年上升。肛瘘是一种常见的肛门直肠疾病。由于它本身无法治愈,手术已成为主要的治疗方法。由于伤口位置和生理结构的特殊性,伤口容易被细菌污染,因此术后换药对伤口愈合起着决定性作用。现代西方医学和传统中医分别有不同的换药方法。本文综述了肛瘘术后常用的中西医换药方法。
{"title":"Research Progress of Traditional Chinese and Western Medicine Dressing Change on Wound Healing After Anal Fistula Surgery","authors":"Xiaona Miao, Jia-min Hang","doi":"10.26689/jcnr.v7i3.4877","DOIUrl":"https://doi.org/10.26689/jcnr.v7i3.4877","url":null,"abstract":"With the change of people’s lifestyle and diet, the incidence of anorectal diseases is increasing year by year. Anal fistula is a common anorectal disease. Because it cannot heal by itself, surgery has become the main treatment method. Due to the particularity of wound location and physiological structure, the wound is easily contaminated by bacteria, so dressing change after surgery plays a decisive role in wound healing. Modern western medicine and traditional Chinese medicine have different dressing changing methods respectively. In this paper, we reviewed commonly used dressing changing methods of traditional Chinese and western medicine after anal fistula surgery.","PeriodicalId":64151,"journal":{"name":"临床护理研究","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44869590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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临床护理研究
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